ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (10): 1382-1387.doi:

• 预防兽医 • Previous Articles     Next Articles

Expression of the Capsid Protein of Rabbit Haemorrhagic Disease Virus in Insect Cells and Its Protective Efficacy to Rabbits

WANG Fang1* ,HU Bo1，REN Xue-feng2, FAN Zhi-yu1，YANG Long-sheng1，
XU Wei-zhong1，ZHANG Ze-bin1，HE Kong-ang1   

  1. 1.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China;2. Jiangsu Animal Disease Control Center, Nanjing 210036,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-24 Published:2008-10-24

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Abstract: The objective of this study is to develop genetic engineering vaccine to rabbit haemorrhagic disease. RT-CR was used to amplify the capsid protein VP60 gene of rabbit haemorrhagic disease virus (RHDV). After being cloned and transformed, the recombinant shuttle vector, Bacmid-P60, was constructed and transfected into Sf9 insect cells, then a recombinant baculovirus, named rAcV-ac-P60 was obtained. The identification by IFA, SDS-AGE, Western blotting, HA and HI showed that the recombinant protein of VP60 was expressed in Sf9 insect cells. The expressed protein was applied to immunize three-month-old rabbits that without immunizing with RHD vaccine. The results showed that the antiRHDV antibodies were induced and developed after 21 days of immunization, the anti-RHDV titers in serum ranged from 26 to 27. When challenged with 2 10 HA units of infectious RHDV, the control rabbits died, while the immunized animals survived. The results indicated that the recombinant proteins expressed by recombinant baculovirus could be ideal candidate for RHDV vaccines.