ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (4): 475-480.doi:

• 遗传繁育 • Previous Articles     Next Articles

Expression Analysis of DECR1 Gene in Tissues of Mashen and Large White Pig

LI Bu-gao1, GUO Xiao-hong1, CAO Guo-qing1, WANG Xiao-jing2, GAO Peng-fei1, LIU Hong3,ZHOU Zhong-xiao1*   

  1. 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University,Taigu 030801, China; 2. Shanxi Livestock and Poultry Breeding Station, Taiyuan 030000, China; 3. Datong Pig Breeding Farm,Datong 037000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

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Abstract: The purpose of this study was to analyze DECR1 mRNA and protein expression profiles of Mashen and Large White pigs in different tissues, and evaluate the potential relationships between its relative expression and lipid metabolism. DECR1 expression profiles in nine tissues (liver, kidney, spleen, lung, small intestine, subcutaneous adipose, stomach, longissimus dorsi, heart) of Mashen and Large White pigs were examined by quantitative realtime PCR and Western blot, respectively. The results indicated that, by real-time PCR, DECR1 had the tissue-and speciesspecific in mRNA expression, but highly in liver, subcutaneous adipose and heart. The DECR1 mRNA expression in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig (P<0.01). The results showed that, by Western blot, the distribution of DECR1 in tissues were significantly different, but highly in subcutaneous adipose, liver and small intestine. Moreover, the expression of DECR1 in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig (P<0.05). It was inferred that DECR1 gene may be related to regulatory mechanism of lipid metabolism.