ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (4): 468-474.doi:

• 遗传繁育 • Previous Articles     Next Articles

A1116T SNP Functional Analysis of Porcine TLR3 Gene

XING Min-wei1, ZHAI Chun-yuan2, LI Hai-tao2, YANG Xiu-qin2*   

  1. 1. College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China；2. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

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Abstract: In order to reveal the biological role of nonsynonymous SNP A1116T of porcine TLR3 Gene and to investigate the relationship of the SNP with disease resistance/susceptibility, the study was designed to construct eukaryotic expression vector of two types of alleles by splicing overlap extension PCR and site-directed mutation methods, to analyze the function of the two alleles in signal transduction in cultured PK-15 cell by dual-luciferase reporter assay and Real-time PCR methods. Two types of eukaryotic expression vectors of porcine TLR3 were successfully constructed. The dual-luciferase reporter assay demonstrated that the mutant type TLR3 allele was defective for activating TLR3-dependent reporter constructers. The real-time PCR analysis showed that the mutant type was reduced in inducing IL-6 transcription. The results indicate that the mutation influences the signal transduction ability of porcine TLR3.