ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (12): 1377-1382.doi:

• 研究简报 • Previous Articles     Next Articles

SNP Detection of Intron 1 of PRL Gene and Its Associations with Egg-laying Traits in Gaoyou Duck

ZHANG Tang-jie;LI Hui-fang;CHEN Kuan-wei;ZHAO Yong-gao;CHANG hong;XUE Min-kai;ZHANG Jing-xin   

  1. 1. College of Veterinary,Yangzhou University,Yangzhou 225009,China;2.Institute of Poultry Science, Chinese Academy of Agricultural Sciences, Yangzhou 225003,China;3.Jiangsu Gaoyou Duck Group, Gaoyou 225601, China; 4.College of Animal Science and Technology, Yangzhou University,Yangzhou 225009,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

PDF (PC)

717

Abstract: In present study, SNP of prolactin(PRL) gene intron 1 of Gaoyou duck were detected by PCR-RFLP and DNA sequencing. The correlation analysis between the polymorphism of PRL gene intron 1 and egg-laying traits was carried out. The results indicated that there were two Dra I digestion sites in intron 1 of PRL gene, but only one was polymorphic. A T/C mutation at the position of the 1 326 bp of the PRL gene was found by DNA sequencing, and resulted in 3 genotypes (AA,AB and BB). The frequencies of genotype BB and allele B were the highest. The least square analysis showed that the BB ducks had higher egg weight at the age of 30 weeks than AB ducks (P<0.01), the proportion of double-yolk of the AB ducks was higher than that of BB ducks (P<0.05), but there was not significant difference in egg number, the longest days of continual laying and the body weight at first laying egg among 3 genotypes.