ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (8): 781-785.doi:

• 遗传繁育 • Previous Articles     Next Articles

SNP Detection and Haplotype Analysis on Exon 16 of Chicken lmbr1

HUANG Yan-qun;CHEN Wen;DENG Xue-mei;QIU Xiang-pin;LI Ning;WU Chang-xin   

  1. 1 College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002,China; 2 State Key Laboratory of Biotechnology, China Agricultural University, Beijing 100094,China; 3College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014,China;4 College of Animal Science and Technology, China Agricultural University, Beijing 100094,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

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Abstract: Exon16 is the biggest exon of chicken lmbr1,here we conduct the SNP detection and haplotype analysis on Silkies and White Plymouth Rock broiler. It shows that the distribution of exon 16 PCR-SSCP genotypes had clear difference between Silkies and White Plymouth Rock broiler ,4 variations were detected by sequencing from 24 individuals, the distribution of allele at T32C site had clear difference between Silkies and White Plymouth Rock broiler too, all the individuals of Silky have 32T allele(in homologous TT or heterozygous TC),while all White Plymouth Rock are homologous CC at T32C site. 5 haplotypes were detected from 24 individuals, the distribution of haplotypes between two breeds also had clear difference,hap1 and hap2 were the special haplotypes of Silkies, while hap5 was the special haplotype of White Plymouth Rock broiler, the hap3 and hap4 were mainly detected from White Plymouth Rock broiler, which were rare in Silkies.