新疆野生荒漠肉苁蓉粗多糖对口蹄疫疫苗免疫小鼠的佐剂效应

doi:10.11843/j.issn.0366-6964.2017.08.019

Abstract

Abstract:

The aim of this study was to investigate the adjuvant effect and safety of wild Cistanche deserticola Y. C. Ma crude polysaccharides (WCDCP) on mice immunized with foot and mouth disease (FMD) vaccine. ICR mice were subcutaneously administrated twice with different doses of WCDCP co-administered with FMDV inactivated antigen or FMD vaccine, respectively. Antibody levels of IgG, IgG₁ and IgG_2a in serum were detected by ELISA. The expression levels of CD4⁺CD44⁺ and CD8⁺CD44⁺ T cells in lymph nodes were tested by flow cytometry. The result showed that medium and high dose of WCDCP could significantly enhance antibody level of FMDV-specific IgG in mice with FMDV inactivated antigen (P<0.01). Medium dose of WCDCP could enhance antibody level of FMDV-specific IgG, IgG₁ and IgG_2a in mice with FMDV vaccine (P<0.05), and long-term antibody level could be elicited. The expression levels of CD4⁺CD44⁺ and CD8⁺CD44⁺ T cells in lymph nodes was significantly higher than that in control group (P<0.05). WCDCP had no effect on the growth of mice. In conclusion, WCDCP can enhance FMDV-specific antibody levels over a long period, significantly enhance the T cell immune response and have no side effects. Therefore, WCDCP can be used as a safe candidate for FMD vaccines.

CLC Number:

S853.31

BA Xue-li, ZHANG Ai-lian, HUANG Jiong, CAO Hui, ZHAO Bing, WANG Dan-yang. Adjuvant Effect of Xinjiang Wild Cistanche deserticola Y.C.Ma Crude Polysaccharides on Foot-and-mouth Disease Vaccines in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1535-1542.

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Adjuvant Effect of Xinjiang Wild Cistanche deserticola Y.C.Ma Crude Polysaccharides on Foot-and-mouth Disease Vaccines in Mice

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