玉米秸秆不同部位的蛋白质营养价值与其分子结构相关关系的研究

doi:doi: 10.11843/j.issn.0366-6964.2016.08.009

Abstract

Abstract:

The objective of this study was to determine whether there was a relationship between protein nutritive values and protein molecular structures at different sections of corn stover.The protein chemical profiles (CP，NDICP，ADICP，NPN，SCP) and CNCPS protein sub-fractions (PA，PB1，PB2，PB3，PC) at different sections of corn stover (ear husk，leaf blade，leaf sheath，stem rind and stem pith) were determined by conventional chemical analysis method and the Cornell Net Carbohydrate and Protein System (CNCPS)；and the spectral parameters of protein molecular structures (including Amide I，Amide II，α-helix，β-sheet) were also analyzed by using the Fourier Transform Infrared Spectroscopy (FTIR).Then the relationship between them was evaluated in this study.The results showed that there were significant differences in the protein nutritive values at different sections of corn stover (P<0.05).The leaf blade had the highest level in CP and NDICP (8.78% and 3.11%，respectively).The sub-fractions such as PA，PB3 and PC were also significantly altered at different sections of corn stover(P<0.05).By FTIR scanning，the protein molecular structures significantly differed among all corn stover sections(P<0.05).Subsequently，obvious correlations were found between protein chemical profiles，protein sub-fractions and protein molecular structures(P<0.05).The content of CP (R ²=0.804 7)，NDICP (R ²=0.949 4)，ADICP (R ²=0.600 9) and PC (R ²=0.647 1) might be predicted by using the peak height ratio of Amide I to Amide II and the peak height ratio of α-helix to β-sheet and peak value of Amide II.It could be concluded that both protein nutritive values and the protein molecular structures were significantly different at different sections of corn stover；and strong relationships between nutritive values and spectral parameters were also observed in this study.

CLC Number:

S513
S501

DING Xue，ZHANG Xing-yi，HAO Xiao-yan，WANG Xiao-fan，XUE Shi-chong，WANG Ming-jun，LI Zhong-yu，ZHANG Yong-gen，XIN Hang-shu. Protein Molecular Structure in Relation to Protein Nutritive Values at Different Sections of Corn Stover[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1592-1600.

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Protein Molecular Structure in Relation to Protein Nutritive Values at Different Sections of Corn Stover

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