表没食子儿茶素没食子酸酯对育肥猪骨骼肌纤维类型的影响

doi:doi: 10.11843/j.issn.0366-6964.2016.08.008

Abstract

Abstract:

The aim of this study was to investigate the effects of Epigallocatechin Gallate (EGCG) on muscle fiber types of finishing pigs.One-hundred eighty crossbred finishing pigs (Duroc × Yorkshire × Landrace，156-day-old) with an average body weight of (85.02 ± 1.15) kg were selected for this study.According to the consistent principle of weight and sex ratio between groups，pigs were randomly divided into 3 groups，and each group with 6 repeats (10 pigs/repeat(5 male，5 female)).Control group was fed with basal diet，treatment groups were fed with basal diet supplemented with 0.025% and 0.05% EGCG，respectively.The results showed that：1) 0.025% EGCG could significantly decrease the activity of LDH，the protein level of MyHCⅠ，PGC-1α，mtTFA and mRNA expression of MyHCⅠ， Tnnt 1，Cytc and COXⅣ in pigs longissimus dorsi (P<0.05)；in psoas major muscle，the activity of LDH and SDH and mRNA expression of MyHC Ⅰwas reduced，the proportion of typeⅠmuscle fiber and mRNA expression of MyHCⅡa，MyHCⅡx，MyHCⅡb were upregulated (P<0.05).2) In longissimus dorsi，0.05% EGCG treatment could significantly decrease the activity of LDH and SDH (P<0.05)，the level of ROS (P<0.05)，and activity of AMPK，the level of protein and mRNA expression of MyHCⅠ，PGC-1α，mtTFA and NRF-1 (P<0.05).While，in psoas major muscle，0.050% EGCG treatment reduced the activity of LDH and SDH (P<0.05)，the level of ROS (P<0.05)，and the protein level and mRNA expression of MyHCⅠ，inhibited the phosphorylation of AMPK and protein expression of NRF-1 (P<0.05)，but the proportion of typeⅠmuscle fiber was upregulated.In conclusion，the effect of EGCG on longissimus dorsi and psoas major of pigs might be completed by reducing ROS level，thus decreasing the AMPK activity and the expression of PGC-1α，eventually reducing the formation of slow-twitch muscle fibers and mitochondria biosynthesis.

CLC Number:

S828
S821.2

WANG Li-na，WANG Zhen，PENG Jian-long，WANG Jian-bing，YANG Ke-lin，SHU Gang，WANG Song-bo，ZHU Xiao-tong，GAO Ping，JIANG Qing-yan. Effects of EGCG on Muscle Fiber Types of Finishing Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1581-1591.

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URL: https://www.xmsyxb.com/EN/doi: 10.11843/j.issn.0366-6964.2016.08.008

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Effects of EGCG on Muscle Fiber Types of Finishing Pigs

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