ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (1): 48-54.doi: 10.11843/j.issn.0366-6964.2016.01.007

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Association between Polymorphism of STAT1 Gene and Milk Production Traits in Buffalo (Bubalus bubalis)

DENG Ting-xian,PANG Chun-ying*,ZHU Peng,DUAN An-qin,LU Xing-rong,YANG Bing-zhuang,LIANG Xian-wei*   

  1. (Key Laboratory of Buffalo Genetics,Breeding and Reproduction Technology,Ministry of Agriculture and Guangxi,Buffalo Research Institute,Chinese Academy of Agricultural Sciences,Nanning 530001,China)
  • Received:2015-01-19 Online:2016-01-23 Published:2016-01-23

Abstract:

This study aimed to identify single nucleotide polymorphism (SNP) of the buffalo STAT1 gene,investigate population genetics characters of SNPs and find molecular markers for milk production traits in Chinese buffaloes.The polymorphisms of STAT1 gene were detected by PCR and sequencing methods.Population genetic polymorphisms for 357 buffaloes were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology,and the associations analysis between genotypes and milk production traits was performed by the SAS (9.3) software with generalized linear model.The results showed that the total of 3 SNPs were detected on the 5′UTR,exon10 and 3′UTR in STAT1 gene,namely g.68G>A,g.986 G>T and g.2881 C>A,respectively.Association analysis indicated that 305-day milk yield and fat percentage were significantly associated with the g.986 G>T of STAT1 gene (P<0.05).The results of Bonferroni t-test revealed that 305-day milk yield of individuals with TT and GG genotypes were significantly higher than those with GT genotypes (P<0.05),and fat percentage of individuals with TT genotype were significantly higher than those with GG and GT genotypes (P<0.05).And the 305-day milk yield tended to increase from first to later parities,reaching the maximum at the 3rd or 4th calving,indicating that parity was one of factors affecting the milk production of buffalo.Our findings in this study implied that the g.986 G>T of STAT1 gene might be a candidate molecular marker for milk production traits in buffalo,which provide a foundation for studying the marker-assisted selection programs in Chinese buffaloes.

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