基于GC-MS策略的奶牛热应激血液代谢组学研究

doi:10.11843/j.issn.0366-6964.2015.08.011

Abstract

Abstract:

The study aimed to reveal the effects of heat stress on dairy cow blood metabolites and its metabolic pathways in a systematic way.According to the criteria for evaluating cow heat stress，10 high milk yield Holstein cows in heat stress status were selected as heat stress group，these cows were regarded as recovery group again until their rectal temperature and respiratory rate returned to the normal range at next morning.Blood samples of each group were collected via tail vein and the plasm were separated.And then we developed and applied a gas chromatography-mass spectrometry GC-MS metabolomics protocol combined with pattern recognition approaches to search differential metabolites between the heat stress and recovery groups，and the differential metabolites were analyzed by KEGG for reconstructing the metabolic pathway and functional analysis.8 metabolites were selected as heat stress potential biomakers and indentified.These biomarkers included alpha-linolenic acid，linoleic acid，lactate，D-glucose，glycerol，hexadecanoic acid，glycocholate and β-Hydroxybutanoate.The content of lactate in heat stress status increased，the other metabolites decreased.Functional pathway analysis revealed that heat stress aggravated the state of energy negative balance in cow，and the lactating dairy cow blood metabolically responded to heat stress through increasing β-oxidation of fatty acid and glycerol metabolic and decreasing in glycolosis activity accompanied with liver dysfuction.The study may provide a strong evidence for further researching the physiological mechanism of heat stress cow.

CLC Number:

S815.4

GUO Yan-sheng，JIA Qi-peng，TAO Jin-zhong. Blood Metabolomic Studies of Heat Stress Cow with GC-MS[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1356-1362.

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Blood Metabolomic Studies of Heat Stress Cow with GC-MS

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