新疆阿拉山口口岸地区鼠类棘球蚴感染情况调查

doi:10.11843/j.issn.0366-6964.2015.06.017

Abstract

Abstract:

The infectious status of echinococcosis in wild rodents was investigated at different areas of Alashankou Port.The wild rodent samples were collected from rural，suburb and urban area of Ala-shankou Port during 2013-2014.Then，pathological sections of liver were prepared and stained with H&E；the genomic DNA of livers and cysts were extracted.Cytochrome C oxidase 1 (Cox1)，NADH dehydrogenase subunit 1 (ND1) in Echinococcus mitochondria were amplified from the DNA and sequenced.Results were as follows：eight samples with cyst were found in the process of autopsy，and the positive rate was 2.08%.Ten positive samples were detected positive by PCR，and the positive rate was 2.60%.The analysis of Blast showed that 8 samples of our sequences were clustered with that of Echinococcus sp. (Accession No.JQ 690286.1)，and their identities were 85%.The remaining 2 samples of our sequences were clustered with that of Echinococcus multilocularis (Accession NO：AB 720065.1)，and their identities were 100%.The results of statitical analysis conveyed us the infection rates of echinococcosis at rural and suburb area were 2.74% and 2.38%，respectively，while no positive sample was detected at urban area.The results presented here demonstrate that the wild rodents at rural area of Ala-shankou Port showed the highest infection rate，and its susceptible host was the Rhombomys opimus.

CLC Number:

S853.734

XIAO Yun-xia，XU Xin-long，YIN Xiao-ping，WANG An-dong，YAN Zun-xiang，DUAN Cheng-ren，XU Jun. Investigation on Echinococcosis in Wild Rodents at Ala-shankou Port[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1004-1010.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.06.017

https://www.xmsyxb.com/EN/Y2015/V46/I6/1004

References

［1］XIAO N，QIU J，NAKAO M，et al.Echinococcus shiquicus，a new species from the Qinghai-Tibet plateau region of China：discovery and epidemiological implications［J］.Parasitol Int，2006，55(Suppl)：233-236.
［2］MANDAL S，MANDAL D M.Human cystic Echinococcosis：epidemiologic，zoonotic，clinical，diagnostic and therapeutic aspects［J］.Asian Pac J Trop Med，2012，5(4)：253-260.
［3］曾祥嫚，官亚宜，伍卫平.棘球蚴病的流行病学分布特征［J］.中国人兽共患病学报，2014，30(4)：413-417.
ZENG X M，GUAN Y Y，WU W P.Epidemiological distribution characteristics of echinococcosis［J］. Chinese Journal of Zoonoses，2014，30(4)：413-417.(in Chinese)
［4］MCMANUS D P，ZHANG W，LI J，et al.Echinococcosis［J］.Lancet，2003，362(9392)：1295-1304.
［5］齐颜凤，伍卫平.棘球蚴病流行病学研究进展［J］.中国寄生虫学与寄生虫病杂志，2013，31(2)：143-148.
QI Y F，WU W P.Progress on the epidemiology of echinococcosis［J］.Chinese Journal of Parasitology and Parasitic Diseases，2013，31(2)：143-148.(in Chinese)
［6］史大中.中国囊性包虫病的地理分布［J］.地方病通报，2000，15(1)：74-75.
SHI D Z.Geographical distribution of hepatic cystic echinococcosis in China［J］.Endemic Diseases Bulletin，2000，15(1)：74-75.(in Chinese)
［7］倪兴维，闫鸿斌，娄忠子，等.野生动物棘球绦虫感染检测方法的研究进展［J］.中国兽医科学，2012，42(1)：100-106.
NI X W，YAN H B，LOU Z Z，et al.Progress in the detection methods of wild animals infected with Echinococcus species［J］.Chinese Veterinary Science，2012，42(1)：100-106.(in Chinese)
［8］汪明.兽医寄生虫学［M］.3版.北京：中国农业出版社，2003：104-124.
WANG M.Veterinary parasitology［M］.3rd edition.Beijing：China Agriculture Press，2003：104-124.(in Chinese)
［9］赵天飙.大沙鼠种群空间分布格局、栖息地选择及种群动态的研究［D］.呼和浩特：内蒙古大学，2006.
ZHAO T B.Studies on spatial distribution pattern，habitats selection and dynamic of Rhombomys opimus population ［D］.Hohhot：Inner Mongolia University，2006.(in Chinese)
［10］郭刚，尹小平，张杰，等.阿拉山口口岸周边野外区鼠情监测报告［J］.口岸卫生控制，2008，13(5)：42-45.
GUO G，YIN X P，ZHANG J，et al.Investigation on rodents and fleas in the area around Ala-shankou Port［J］.Port Health Control，2008，13(5)：42-45.(in Chinese)
［11］OIE International Committee.OIE Terrestrial Manual，CHAPTER2.1.4.Echinococcosis/Hydatidosis［M］.2008：175-189.
［12］TRACHSEL D，DEPLAZES P，MATHIS A.Identification of taeniid eggs in the faeces from carnivores based on multiplex PCR using targets in mitochondrial DNA［J］.Parasitology，2007，134(Pt 6)：911-920.
［13］ABBASI I，BRANZBURG A，CAMPOS-PONCE M，et al.Copro-diagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence［J］.Am J Trop Med Hyg，2003，69(3)：324-330.
［14］李晓军，王文明，赵莉，等.新疆包虫病流行现状与防控对策［J］.草食家畜，2012(4)：47-52.
LI X J，WANG W M，ZHAO L，et al.The epidemic and control situation of hydatid disease in Xinjiang［J］. Grass-Feeding Livestock，2012(4)：47-52.(in Chinese)
［15］李宏民，屈亚锦，李娟，等.绵羊棘球蚴病的病原学鉴定及组织病理学观察［J］.中国兽医科学，2012，42(9)：949-953.
LI H M，QU Y J，LI J，et al.Pathological identification and histopathological observation of ovine echinococcosis［J］.Chinese Veterinary Science，2012，42(9)：949-953.(in Chinese)
［16］贾红，刘丹，侯绍华，等.羊细粒棘球蚴病抗体间接ELISA检测方法的建立［J］.畜牧兽医学报，2011，42(1)：65-70.
JIA H，LIU D，HOU S H，et al.Development of an indirect ELISA for detection of sheep antibodies against Echinococcosis granulosa［J］.Acta Veterinaria et Zootechnica Sinica，2011，42(1)：65-70.(in Chinese)
［17］贾万忠，闫鸿斌，郭爱疆，等.带科绦虫线粒体基因组全序列分析研究进展［J］.中国人兽共患病学报，2010，26(6)：596-600.
JIA W Z，YAN H B，GUO A J，et al.Progress on complete sequence of mitochondrial genome from Taeniidae［J］.Chinese Journal of Zoonoses，2010，26(6)：596-600.(in Chinese)
［18］尹小平，彭定希，骄娃，等.新疆中哈边境地区大沙鼠疫源地种群繁殖调查［J］.中国国境卫生检疫杂志，2011，34(5)：351-358.
YIN X P，PENG D X，JIAO W，et al.Investigation and research of Rhombomys Opimus on population propagation and influence factors at Sino-kazakh border area［J］.Chinese Frontier Health Quarantine，2011，34(5)：351-358.(in Chinese)
［19］杨光友，张志和.野生动物寄生虫病学［M］.北京：科学出版社，2013.
YANG G Y，ZHANG Z H.Parasitic diseases of wildlife［M］.Beijing：Science Press，2013.(in Chinese)
［20］唐崇惕，康育民，崔贵文，等.我国内蒙古大兴安岭北麓泡状肝包虫种类的研究Ⅲ.苏俄棘球绦虫(Echinococcus russicensis sp.nov.)［J］.中国人兽共患病学报，2007，23(10)：957-963.
TANG C T，KANG Y M，CUI G W，et al.Studies on the alveolar Echinococcus species in northward daxingan mountains，Inner Mongolia，China.Ⅲ.Echinococcus russicensis sp.nov.［J］.Chinese Journal of Zoonoses，2007，23(10)：957-963.(in Chinese)

Investigation on Echinococcosis in Wild Rodents at Ala-shankou Port

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments