褪黑素受体在未妊娠和妊娠牦牛子宫表达的研究

doi:10.11843/j.issn.0366-6964.2015.02.009

Abstract

Abstract:

To better understand the role of melatonin receptor (MT1) during pregnancy of yak，the expression level of MT1 protein and MT1 mRNA in uterus of yak (Bos grunniens)during pregnant and nonpregnant stage was determined using Streptavidin-Peroxidase (S-P) immunohistochemical method and real-time PCR，respectively.The results showed that MT1 protein expressed in stromal cells and myometrial smooth muscle cells during pregnancy and nonpregnant stage (P>0.05).The expression level of MT1 protein in endometrial blood vessel and vascular smooth muscle had no significant difference on 28-35 day of pregnancy compared with that of non-pregnant yak，however，it was increasing with the stage of pregnancy (P<0.05).Meanwhile，MT1 protein expression was not significant different on 28-35 day of pregnancy in gland epithelium，but had dramatic increases on 50-60 day of pregnancy (P<0.05) and 90-100 day of pregnancy (P<0.01).In surface epithelium cells，the expression of MT1 protein was lower on 28-35 day of pregnancy than that of nonpregnant stage (P<0.05) and had significant decreases on 50-60 day of pregnancy (P<0.01) and 90-100 day of pregnancy (P<0.01).In addition，MT1 mRNA expression was lower in pregnant uterus than that of nonpregnant stage (P<0.01).The results indicated that the decreased MT1 mRNA expression in uterus during pregnancy might be associated with gland epithelium cell function for maintenance of pregnancy.

CLC Number:

S823.8⁺5.2

HUO Sheng-dong，YUAN Qi-jian，LU Jian-xiong，ZANG Rong-xin，YANG Ju-tian，ZHANG Tao-jie，Ayimuguli，LIU Jun-lin，MA Zhong-ren，LONG Run-jun. Melatonin Receptor Expression in Uterus during Pregnancy，Nonpregnancy of Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 239-244.

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Melatonin Receptor Expression in Uterus during Pregnancy，Nonpregnancy of Yak

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