SIRT1和SIRT2基因多态性及合并基因型与秦川牛肉用性状的关联分析

doi:10.11843/j.issn.0366-6964.2015.10.006

Abstract

Abstract:

This study aimed to explore the association of single nucleotide polymorphism(SNP) of SIRT1 and SIRT2 genes with Qinchuan cattle meat quality traits，so as to search for molecular markers related with meat quality traits.DNA seuqencing and PCR-PFLP methods were applied to detect the genotype polymorphisms of SIRT1 and SIRT2 genes in Qinchuan cattle populations，and analyze the associations of single and combined genotypes with meat quality traits of Qinchuan cattle.Results showed that 2 SNPs(G25764A and A25846G) were detected in 3′-untranslated region of SIRT1 gene，and 2 mutants(C19501T and C19518T) in 3′-untranslated region of SIRT2 gene were identified，too.Association analysis indicated that，in Qinchuan cattle population with 505 individuals，at G25764A locus，individuals with AA genotype exhibited very significantly higher loin-eye area than those with AB genotype(P<0.01).At A25846G locus，the loin-eye area of animals with AA genotype was significantly higher than that of animals with AB genotype(P<0.05) and very significantly higher than that of animals with BB genotype(P<0.01).Moreover，the C19501T locus significantly affected loin-eye area and intramuscular fatty content(P＜0.05)，and AA was the preferable genotype.Besides，at C19518T locus，individuals with the BB genotype showed significantly higher backfat thinckness compared to those with the AA genotype(P<0.05).The optimal combined genotype of SIRT1-SIRT2 was AB-BB-AA-BB，which was more effective on meat quality traits than other combined genotypes(P＜0.01 or P＜0.05).These results suggested that the single nucleotide polymorphisms and combined genotypes of both SIRT1 and SIRT2 genes affected the meat quality traits of Qinchuan cattle，and they could be used as candidate genes for breeding Qinchuan cattle with better meat quality traits.

CLC Number:

S823
S813.3

GUI Lin-sheng，ZAN Lin-sen，WANG Hong-bao，WANG Hong-cheng. Effects of Single and Combined Genotypes of SIRT1 and SIRT2 Genes on Meat Quality Traits in Qinchuan Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1741-1749.

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Effects of Single and Combined Genotypes of SIRT1 and SIRT2 Genes on Meat Quality Traits in Qinchuan Cattle

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