绵羊IGF-Ⅰ基因的表达及其SNPs与生长性状的相关分析研究

doi:10.11843/j.issn.0366-6964.2013.09.005

Abstract

Abstract:

This experiment was conducted to study the expression patten of IGF-Ⅰgene regulating growth and development of sheep and correlation of its SNPs with growth traits in sheep. The IGF-1 gene expression change of IGF-Ⅰin brain,musle,skin,liver and heart were detected at six growth stages(15,60,105,150,195,240 days) by qRT-PCR approach and the correlation of SNPs of IGF-Ⅰgene with growth traits were analyzed by SSCP method in Liangshan Semi-wool sheep population. The results showed that the first inflection point of the expression of IGF-Ⅰgene in musle, skin, brain, liver and heart tissues was at 105 day and the second inflection point was at 195 day without the liver tissue. From 15 to 105 day, the expression of IGF-1 gene in musle, brain and liver tissues decreased at 60 day, and then increased at 105 day, however, it is not significantly different at 15 and 60 day, and increased significantly at 105 day in skin and heart tissues.After 105 day, the expression of IGF-Ⅰ were degraded in all of the tissues at 150 day,and the expression in liver tissue was decreased gradually from 105 to 240 day, but the expression in skin and brain tissues were higher at 195 day than that at 150 and 240 day, and no difference at among 150, 195 and 240 day in musle and heart tissues.The two SNPs which were detected with 587 Liangshan Semi-wool sheep were moderate polymorphism. The SNP of the P-1 primer affected significantly birth weight(P<0.05) and the SNP of the P-2 primer affected significantly weaning weight and the weaning daily gain(P<0.05). The results suggest that the expression of IGF-Ⅰin all tissues have the similar expression patterns,and strong correlation between SNPs of IGF-1 and confirmed the early growth traits provide theoretical basis for the growth regulation and can be applied to assisted selective breeding with the SNPs in Liangshan Semi-wool sheep.

CLC Number:

S826
S813.3

ZHOU Ming-liang, ZHANG Xiang-yu, QI Song-zhi, WANG Qi, WU Deng-jun,YANG Ping-gui. Expression and Association between SNPs and Growth Traits of IGF-Ⅰ Gene in Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1363-1370.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/ 10.11843/j.issn.0366-6964.2013.09.005

https://www.xmsyxb.com/EN/Y2013/V44/I9/1363

References

［1］VERHAEGHE J, VAN B R, VAN H E, et al. C-peptide, insulin-like growth factors I and II, and insulin-like growth factor binding protein-1 in umbilical cord serum:Correlations with birth weight［J］. Am J Obst Gynecol, 1993, 169(1): 89-97.

［2］MUSHTAQ T, BIJMAN P, AHMED S F, et al. Insulin-like growth factor-I augments chondrocyte hypertrophy and reverses glucocorticoid-mediated growth retardation in fetal mice metatarsal cultures［J］. Endocrinology, 2004, 145(5): 2478-2486.

［3］JOSRPH D A, YE P. Expanding the mind: insulin-like growth factor I and brain development［J］. Endocrinology, 2008, 149(12): 5958-5962.

［4］BENNETT A K, HESTER P Y, SPURLOCK D E. Polymorphisms in vitamin D receptor, osteopontin, insulin-like growth factor 1 and insulin, and their associations with bone, egg and growth traits in a layer--broiler cross in chickens［J］. Anim Genet, 2006, 37(3): 283-286.

［5］JOHNSTON L B, DAHLGREN J, LEGER J, et al. Association between insulin-like growth factor I (IGF-I) polymorphisms, circulating IGF-I, and pre- and postnatal growth in two European small for gestational age populations［J］. J Clin Endocrinol Metab, 2003, 88(10): 4805-4810.

［6］ZHOU H, MITCHELL A D, MCMURTRY J P, et al. Insulin-like growth factor-I gene polymorphism associations with growth, body composition, skeleton integrity, and metabolic traits in chickens［J］. Poult Sci, 2005, 84(2): 212-219.

［7］TAYLOR J F, PORTER M J, BROMAGE N R, et al. Relationships between environmental changes, maturity, growth rate and plasma insulin-like growth factor-I (IGF-I) in female rainbow trout［J］. Gen Comp Endocrinol, 2008, 155(2): 257-270.

［8］AMILLS M, JIMENEZ N, VILLALBA D, et al. Identification of three single nucleotide polymorphisms in the chicken insulin-like growth factor 1 and 2 genes and their associations with growth and feeding traits［J］. Poult Sci, 2003, 82(10): 1485-1493.

［9］MAJ A, SNOCHOWSKI M, SIADKOWSKA E, et al. Polymorphism in genes of growth hormone receptor (GHR) and insulin-like growth factor-1 (IGF1) and its association with both the IGF1 expression in liver and its level in blood in Polish Holstein-Friesian cattle［J］. Neuro Endocrinol Lett, 2008, 29(6): 981-989.

［10］周明亮, 吴登俊，张翔宇. 绵羊3号染色体的遗传连锁图谱构建及QTL定位［J］. 遗传, 2007, 29(12): 1475-1482.

［11］BRIAN P D, EWEN F K, MIKHAIL N, et al. Using comparative genomics to reorder the human genome sequence into a virtual sheep genome［J］. Genome Biol, 2007, 8(7): R152.

［12］BOTSTEIN D, WHITE R L, SKOLNICK M. Construction of a genetic linkage map in man using restriction fragment length polymorphism［J］. Am J Human Genet, 1980, 32(3): 314-331.

［13］VANDESOMPELE J, DE PRETER K, PATTYN F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes［J］. Genome Biol, 2002, 3(7): 34-42.

［14］刘国庆, 黄治国, 刘振山, 等. 羔羊肝脏IGF-I和IGF-IR基因表达的发育性变化研究［J］. 中国农业科学, 2006, 39(12): 2577-2581.

［15］贾斌, 席继峰, 张苏云, 等. 绵羊皮肤中GHR、IGF-I和IGF-IR基因表达的发育性变化及品种特点［J］. 遗传, 2006, 28(9): 1078-1082.

［16］XIAN C J, SHOUBRIDGE C A, READ L C. Degradation of IGF-1 in the adult rat gastrointestinal tract is limited by a specific antiserum or the dietary protein casein［J］. J Endocrinol, 1996, 146: 215-225.

［17］RAO R K, PHILIPPS A F, WILLIAMS C S, et al. Luminal stability of insulin-like growth factors 1 and 2 in developing rat gastrointestinal tract［J］. J Pedi Gastroenterol Nutr, 1998, 26: 179-185.

[1]	LUAN Zhao-jin，HE Jian-ning，CHENG Ming，LIU Kai-dong，QU Xu-xian，LIU Nan. Association of the MyoG and PID1 Gene Expression with Intramuscular Fat Content in Sheep Muscles [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1986-1994.
[2]	XUAN Jun-li，MA Xiao-meng，WANG Hui-hua，CAO Jia-xue，WEI Cai-hong，ZHAO Fu-ping，MA You-ji，ZHANG Li，DU Li-xin. Study on Exon Polymorphism of Seasonal Breeding Related Gene TSHR in Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1342-1353.
[3]	DUAN Xin-chong，WEI Yan-hui，LI Yang，LI Xiang-yun，ZHOU Rong-yan，XI Jian-zhong. Analyzing the Differential Expression of microRNAs in Estrus and Diestrus of Small Tailed Han Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1324-1332.
[4]	MA Xiao-meng，XUAN Jun-li，WANG Hui-hua，YUAN Ze-hu，WU Ming-ming，ZHU Cai-ye，LIU Rui-zao，WEI Cai-hong，ZHAO Fu-ping，ZHANG Li，DU Li-xin. TXNRD1 Gene Polymorphisms and Its Association with Growth Traits in Ujumqin Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 909-921.
[5]	YUAN Ze-hu，WANG Hui-hua，HU Shi-jin，ZHU Cai-ye，ZHAO Fu-ping，ZHANG Li，DU Li-xin，WEI Cai-hong. Population Structure Analysis of Chinese Indigenous Sheep by 50K Chip Data [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 899-908.
[6]	CHEN Xiao-yong，SUN Hong-xin，YUAN Ming，KANG Zhen-jiang，DUN Wei-tao，ZHAO Xing-bo. Association Analyses between Mitochondrial Coding Gene Variations and Sheep Litter Size [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 449-456.
[7]	LIU Rui-zao，LIU Zhen，WU Ming-ming，WANG Hui-hua，ZHU Cai-ye，ZHANG Li，ZHAO Fu-ping，WEI Cai-hong，DU Li-xin. Expression Trend of BTG2/3 in the Mid to Late Embryonic Skeletal Muscle of Sheep and the Relationship between BTG2/3 and MSTN [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1916-1923.
[8]	SA Ru-li，MU Qi-er，WANG Cui-fang，BAO Ling-ling，AO Chang-jin，WANG Si-zhen. Effect of Allium Mongolicum Regel Flavonoids on Lymphocyte Proliferation，mRNA Expression of IL-2，IL-4， IFN-γ in Peripheral Blood Lymphocyte of Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1063-1070.
[9]	WANG Pei，FAN Yan-ru，JIN Xin，YANG Yin-feng. Effects of Bacillus subtilis on the Expression of SBD-1 in Cultured Ruminal Epithelial Cells of Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 760-767.
[10]	ZHANG Chun-xiang，GUO Yun-yan，BAI Yuan-sheng,YANG Zi-sen，ZHANG Guo-lin，JIAO Guang-yue，REN You-she. Clone，Bioinformatics Analysis and Expression Characteristics of Ovine GPx5 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1075-1083.
[11]	LIU Qiang，HE Zhi-rui，WANG Yin-long，WU RE lihazi，SAI Wujiafu. Construction Eukaryotic Expression Vector and Its Expression of PLCζ Gene from Chinese Merino Sheep [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 541-546.
[12]	WEI Lin-lin, GAO Jin-sheng, WANG Jing-lin, CHENG Li-fen, QIAO Li-ying, JIA Xia-li, ZHANG Jing, YANG Kai-jie, LIU Jian-hua, LIANG Chen, LIU Wen-zhong. Study on the Cloning and Ontogenetic mRNA Expression of Ovine LPIN1 Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1371-1379.
[13]	RONG En-guang, QIAO Shu-pei, YU Lei, YAN Xiao-hong,YANG Hua, LI Hui, WANG Ning. Cloning and Expression Characterization of Sheep POU2F3 Gene and Its Isoforms in Chinese Merino Skin [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1189-1197.
[14]	LI Da,SUN Wei, SU Rui, ZHANG Zhan-ying, MA Yue-hui, ZHANG You-fa,CHEN Ling, WU Wen-zhong, ZHOU Hong. Molecular Cloning and Construction of Eukaryotic Expression Vector and Mutant Vector of Sheep YAP1 Gene [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1198-1204.
[15]	LIU Bao-feng, ZHOU Sha-sha, WANG Jing-lin, WEI Lin-lin, QIAO Li-ying, LIU Jian-hua, LIANG Chen, LIU Wen-zhong. Differential Expression of Lep and LEPR mRNA in Adipose Tissues of Sheep with Divergent Fat-tails [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1014-1022.

Expression and Association between SNPs and Growth Traits of IGF-Ⅰ Gene in Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments