共轭亚油酸对堆型艾美耳球虫感染鸡十二指肠黏膜免疫和脂质过氧化状态的影响

Abstract

Abstract: The present experiment was conducted to investigate effects of conjugated linoleic acid (CLA) on growth performance, oocyst shedding, duodenumal mucosal immune response and lipid peroxidation of broiler chicks infected with E. aceruvlina. After 21 days of dietary supplementation with either basal diets or 1.5% CLA diets, half of replicates in each treatment were inoculated with 10 000 E. aceruvlina sporulated oocysts. Compared with basal diets, 1.5% CLA diets ameliorated growth inhibition (P<0.01), and decreased oocyst shedding between 6-9 d post-inoculation resulted from E.aceruvlina infection (P<0.01). Whether infection or not, duodenumal intraepithelial lymphocyte （IEL） CD8⁺T lymphocyte subpopulations were significantly higher in chicks fed CLA diet than the controls (P<0.05), but interferon γ （IFNγ） gene expression was not (P>0.05). Dietary CLA notably suppressed the increase of MDA in duodenumal mucosa (P<0.05), and inhibited the decrease of glutathione (GSH) concentration （P<0.01） resulted from E.aceruvlina infection, but didn’t influence the activity of total superoxide dismutase （TSOD） and catalase （CAT）（P>0.05）. Dietary CLA decreased oocyst shedding and ameliorated growth ihibition of broiler chicks resulted from E.aceruvlina infection at least in part through increasing duodenumal IEL CD8⁺T lymphocyte subpopulations and inhibiting the decrease of duodenumal mucosal GSH in broiler chicks.

CLC Number:

S852.7

LIU Yong-Xiang, YANG Jian-Ping, JIANG Dong-Feng, LIU Yan-Li, XU Qiu-Liang. Effects of Dietary Conjugated Linoleic Acid on the Duodenumal Mucosal Immunity
Response and Lipid Peroxidation of Broiler Chicks Infected with E.aceruvlina[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(9): 1463-1470.

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Effects of Dietary Conjugated Linoleic Acid on the Duodenumal Mucosal Immunity
Response and Lipid Peroxidation of Broiler Chicks Infected with E.aceruvlina

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Effects of Dietary Conjugated Linoleic Acid on the Duodenumal Mucosal Immunity Response and Lipid Peroxidation of Broiler Chicks Infected with E.aceruvlina

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Effects of Dietary Conjugated Linoleic Acid on the Duodenumal Mucosal Immunity
Response and Lipid Peroxidation of Broiler Chicks Infected with E.aceruvlina