Abstract: The aim of this study was to study the effect of sex gland hormones on the secretory activity to endometrial cells of goats. Based on the hanging cell coculture insert system, immortalization of goats endometrial epithelial cells (EEC) and stromal cells (ESC) has trained coculture in vitro studies model, the different levels and combinations of progesterone (P4) and estradiol (E2) were added in medium, without or with ESC together, TNFα and TGFβ secretory activity of EEC were determined by ELISA. The results showed that compared with no hormone control group, the secretion of TNFα on EEC apical was enhanced markedly by P4 (P＜0.05) when added alone or together E2 and P4, but not significant on basolateral; TGFβ secretory activity of both EEC apical and basolateral were reduced markedly by different concentrations and combinations of E2 and/or P4 (P＜0.05), but TGFβ on EEC apical was restrained significantly by E2 alone (P＜0.05). About coculture with ESC together, both apical and basolateral of TNFα secretion were repressed significantly by sex hormones E2/P4 (P＜0.05); TGFβ on EEC apical was restrained significantly by E2/P4 (P＜0.05), but not significant on basolateral. Our results indicated that the sex hormones in coculture mode alone or together with ESC, all can regulate the secretion of TNFα and TGFβ with different levels, suggested that ESC play an important regulatory role to the secrete direction and secretion levels of goat EEC when coculture with sex hormones.