Abstract: This experiment was conducted to obtain the coding sequence of Banna Mini-pig Inbred Line (BMI) growth hormone (GH) gene and to study its prokaryotic expression. BMI GH gene sequence was cloned from pituitary by RT-PCR and inserted into pMD 18-T vector for sequencing and bioinformatics analysis. The length of GH cDNA sequence was 690 bp, which contained a CDS of 651 bp. GH CDS encoded 216 amino acids with 26 amino acids of signal peptide. It was found that the GH amino acids sequence shared 100%, 100%, 99%, 99%, 99%, 99%, 99%, 99%, 99%, 99%, 99%, 98% and 98% identity with those of Wuzhishan pig, Ningxiang pig, Tibetan pig, Xiang pig, Dawu pig, Taihu pig, Chenghua pig, Neijiang pig, Rongchang pig, Landrace, Yorkshire, Yanan pig and Duroc pig, respectively. Subcloned the GH encoding mature peptide sequence into the prokaryotic expressing vector pET-32a (+) directionally to constitute the recombinant plasmid pET-32a-GH, then it was transformed into the E.coli Rosseta bacteria for expression under induction of IPTG. SDS-PAGE and Western blotting analysis showed that recombinant plasmid pET-32a-GH was successfully expressed in high level. The fusion protein expressed in form of inclusion body and the molecular weight was approximate 40.6 ku. These results will lay the foundation for further studies of the effects of GH on the dwarfism of Banna Mini-pig Inbred Line.