猪繁殖与呼吸综合征病毒强弱毒株嵌合感染性克隆的构建及鉴定

Abstract

Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of the ongoing “porcine high fever syndrome” in China, is capable of genetic and antigenic mutations at high frequency. How to design vaccine rationally to keep up with the ever-changing prevalent PRRSV variant is of great interest. In this study, based on an infectious cDNA clone of an attenuated TypeⅡ PRRSV strain pAPRRS and the highly pathogenic PRRSV cDNA clone pJX143, we replaced the coding sequence of pAPRRS nsp2 with those of the HP PRRSV to develop a series of chimeric clones. Upon transfection of chimeric clones into MA104 cells, typical PRRSV cytopathic effects were observed. This study provided a valuable tool to develop the chimeric PRRSV as vaccine candidate offering cross-protection to HP PRRSV strains. Furthermore, the infectious chimeric cDNA clone provides a powerful tool to molecular dissection of the mechanism of pathogenesis of the increasing-virulence of the on-going prevalent PRRSV in China.

CLC Number:

S852.659.6

LV Jian, WEI Zu-zhang, GAO Fei, ZHENG Hai-hong, TONG Guang-zhi, YUAN Shi-shan. The Construction of Chimeric Clone of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Attenuated PRRSV Strain and Identification of Chimeric Viruses Rescued[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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The Construction of Chimeric Clone of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Attenuated PRRSV Strain and Identification of Chimeric Viruses Rescued

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