ACTA VETERINARIA ET ZOOTECHNICA SINICA

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Regulation of DNA Methylation during Staphylococcus aureus Mastitis in Dairy Cattle

LIU Li, GAO Xue, GAO Hui-jiang, ZHANG Lu-pei, LI Jun-ya, XU Shang-zhong*   

  1. (Laboratory of Bovine Genetics and Breeding, Institute of Animal Science, Chinese
    Academy of Agricultural Sciences, Beijing 100193, China)
  • Received:2012-02-09 Online:2012-10-25 Published:2012-10-25

Abstract: The aim of this study was to study the change of DNA methylation around STAT5-binding region in the far upstream promoter and its effect on gene expression of the bovine CSN1S1 during Staphylococcus aureus mastitis in dairy cattle. The methylation status of the promoter and the expression of CSN1S1 mRNA were detected by the bisulphite modification technique and the real-time quantitative PCR at 0,12,24,36 and 196 h after intramammary inoculation with Staphylococcus aureus.The result showed that the level of methylation gradually increased with time within 24 h,while the expression of CSN1S1 mRNA were significantly down-regulated in infected mammary tissue(P<0.01), at 36 and 196 h after infection, the methylation status and gene expression levels were no significant difference from that at 24 h.The results suggest that DNA re-methylation of mammary tissue can be caused by Staphylococcus aureus mastitis, DNA re-methylation may be a general regulation of the acute phase of bovine mastitis.

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