ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (7): 945-949.doi:

• 预防兽医 • Previous Articles     Next Articles

Construction of Gene Vaccine Vector Carrying TSOL18 Gene of Taenia solium Oncosphere and Its Immunogenicity

GUO Ai-jiang1,2, CAI Xue-peng2*, FANG Yong-xiang2, JIA Wan-zhong2, LUO Xue-nong2, LIU Hong-xia2   

  1. 1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;2. Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

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Abstract: In this study, recombinant expression plasmid pVAX1/TSOL18 was constructed and then transfected into BHK-21 cell line with liposome 2000. The results of SDS-PAGE, Western blotting and fluorescence antibody assay showed that TSOL18 protein was successfully expressed in BHK-21 cell line and could be recognized by positive serum against cysticercosis.Immunization in mice indicated that the expression vectors could efficiently induce the specific cellular and humoral immunoresponse, which laid a solid foundation for the further development of new vaccines of cysticercosis.