ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (7): 930-934.doi:

• 预防兽医 • Previous Articles     Next Articles

Protective Antigens from Virulent Isolates of Fusobacterium necrophorum FN(A)

MIAO Li-guang1,YANG Fu-he1*,LIU Yan-huan1 ,WANG Zhi-gang1,
LI Yan1, WANG Ke-jian2,XIAO Jia-mei1
  

  1. 1.Institute of Special Wild Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jilin 132109,China;2. Environmental Sciences Research Center, Research Lab of SEDC of Marine Environment, Xiamen University, Xiamen 361005, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

Abstract: A virulent isolate of the bacterium FN(A) was lysed with trypsin. Supernatants and precipitates of the bacterial lysates were used to prepare anti-FN serum by immunizing rabbits. Four immunogenic components were isolated using SDS-PAGE/Western-blot. Immunized rabbits were challenged,and one protective antigen was screened. After antigen isolation and purification, the N-terminal amino acid sequence was determined and an immunological test and hemolysis test were performed. Results suggested that this antigen is either a hemolysin analogue or a new antigenic substance. The antigen was able to induce a protective immune response as well as generate a marked cross-immune reaction among different bacterial strains.