Abstract: In order to develope rapid detection technology for avian influenza virus antigen, a disposable electrochemical immunosensor was constructed by coating the screen-printed carbon electrode with chitosan membrane and HRP-anti-AIV . Cyclic voltammetry was selected as electrochemical means to detect the products of the enzymatic reaction. According to the decrease percentage of reduction current, AIV-Ag could be detected. Under the optimum conditions of immunoreaction and electrochemical detection, AIV-Ag can be rapidly detected by sigmoidal curve method and form a linear calibration between 3.75 to 125 ng/mL with an associated detection limit of 0.56 ng/mL (S/N=3). The immunosensor had acceptable specificity, reproducibility (RSD＜7.10%), stability (the amperometric response was 91.46% of the initial response after two weeks) and accuracy (91.18% of the results obtained by the immunosensor were in agreement with those obtained by ELISA). In addition, four channels screen-printed carbon electrode was employed for fabricating AIV-Ag immunosensor, the results indicated that the four channels immunosensor had good consistency. Therefore, the immunosensor could be hopefully used to detect AIV-Ag rapidly.