ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (10): 1415-1420.doi:

• 基础兽医 • Previous Articles     Next Articles

Effects of Somatostatin Fusion Gene Carried with PLGA Microspheres on Rabbits Growth

DAI Jian-wei2，3, ZHANG Qian-qian4, WANG Yan5， LIU Song-cai3, ZHANG Yong-liang1*   

  1. 1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China; 2.Department of Biochemistry, Guangzhou Medical College, Guangzhou 510182,China;3. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 4. Key Laboratory of Gene Engineering of the Ministry of Education, Biotechnology Research Center, Sun Yatsen University, Guangzhou 510275; China; 5. Veterinary Medicine Department, Agricultural College, Yanbian University, Longjing 133400，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-24 Published:2008-10-24

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Abstract: In this paper, we described the regulation of animal growth using plasmid DNA encoding the SS gene that fused with the hepatitis B surface antigen (HBsAg) gene. The fusion genes were successfully expressed in CHO cells, as proven by RT-PCR and ELISA assayed. Poly D, L-lactide-co-glycolic acid (PLGA) plasmid-encapsulating microspheres were prepared and injected directly into the anterior tibialis of rabbits. Daily weight gain and the levels of insulinlike growth factor-Ⅰ (IGF-Ⅰ), SS, and hepatitis B surface antibody (HBsAb) were monitored. From days 15 to 30 postinjection, the daily weight gain of the treated group was significantly increased (P<0.01) by 1068%. A significant increase in the IGF-Ⅰ concentration and decrease in the SS level of the treated group were also observed. The results indicated that the combination of plasmid-mediated positive immunization against SS led to more robust weight gain in rabbits.