Abstract: Myostatin, a new member of the TGF-β super family, is specifically expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth. In this study, the single nucleotide polymorphisms (SNPs) of Myostatin gene was identified in various goose breeds including Zhedong White geese, Wulong geese, Yangzhou geese, Landish geese, Rheine geese, Huo geese, Jilin White geese, Shitou geese, Wanxi White geese, Zi geese, etc. by PCR-SSCP and DNA sequencing. The results showed that: （1） The 12 pairs of primers for goose Myostatin gene were designed based on goose Myostatin gene sequence. Three SNPs located in the promoter region of the Myostatin (T769G, C543T ) and the intron 1 (A1 632G) were identified in geese Myostatin gene, respectively. （2）Population genetic analyses indicated that frequency of allele A ( P3 ) in promoter region was over 0.77 and higher than that of allele B, which is a dominant allele. Frequency of allele D ( P4 ) among Yangzhou geese, Landish geese, and Shitou geese were higher than that of allele C, and the frequency of allele D were 0.625 0, 0.548 8 and 0.647 4 among the three breeds, respectively. But on the contrary, it presented that frequency of allele C is higher than that of allele D in other breeds.