Abstract: The chicken were immunized orally with NDV strain LaSota vaccine containing two kinds of compound mucosal immune adjuvant(Group adjuvant Ⅰ and Ⅱ) respectively. The CD3+T lymphocytes, intraepithelial lymphocytes(IEL) and IFN-γ mRNA expression of small intestine in chicken were investigated. The results showed that the number of CD3+T lymphocytes and IELs increased during the period of immunity. The number of CD3+T lymphocytes of Group adjuvant Ⅱ were higher than that of Group adjuvant Ⅰ (P<0.05) in duodenum and significantly higher (P<0.01) in jejunum at the 3rd week and 5th week after vaccination. The number of IELs of Group adjuvant Ⅱ were significantly higher than that of Group adjuvant Ⅰ (P<0.01) in Peyer’s Patch at the 7th week after vaccination. The IFN-γ mRNA expression of two compound adjuvant groups decreased after vaccination. The results demonstrated that compound adjuvants could induce high level cellular immune response and promote intestine mucosal immunity in chicken.