ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (9): 999-1002.doi:

• 研究简报 • Previous Articles     Next Articles

Construction of cDNA Expression Library of Eimeria tenella Sporozoites

LIN Qing;CAI Xue-peng;DOU Yong-xi;ZHAI Jun-jun;YAN Hong-bin;
ZHANG Yan-ming;TIAN Guang-fu;JING Zhi-zhong   

  1. 1 College of Animal Science and Technology，Northwest A & F University，Yangling 712100，China；2 Key Laboratory of Veterinary Parasitology of Gansu Province，State Key Laboratory of Veterinary Etiological Biology， Lanzhou Veterinary Research Institute，Chinese Academy of Agricultural Sciences， Lanzhou 730046，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

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Abstract: Total RNA and subsequent mRNA were isolated from E tenella sporozoites A library of Oligo(dT)-primed cDNA with added directional EcoR I /Hind Ⅲ linkers was constructed and ligated to the EcoR I /Hind Ⅲ arms of the screen vector, the recombinant phage DNA was packaged by using PhageMaker packaging extracts, resulting in a primary cDNA library. The library was proved to be a good quality by the control tests. The cloning efficiency was evaluated and the length of the cDNA fragment was assayed by PCR. Using the amplified library as template DNA, a pair of primers were designed according to the sequence of the E. tenella 3-1E, then the gene was amplified by PCR. The results showed that the cDNA expression library of E. tenella sporozoites was constructed and the 3-1E gene was amplified successfully. The capacity of the cDNA library was 4×106 pfu/mL and the length of inserts was about 100-3 000 bp. It is helpful in the further study on screening related genes.