ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (9): 1003-1008.doi:

• 研究简报 • Previous Articles    

Construction of Pig IL-2 Eukaryotic Expression Plasmid pcDNA-IL-2 and Its Adjuvant Effect on pcDNA-PRRSV-SC2-ORF5 Gene Vaccine

Xininigen;CHENG An-chun; WANG Ming-shu;CHEN Xi-wen;DOU Wen-bo;
LI Xue-mei;LIU Wu-mei;ZHANG Ping-ying; LIU Fei; CHEN Xiao-yue   

  1. 1. Sichuan Key Laboratory of Animal Disease and Human Health，Ya’an 625014, China; 2.Research Center of Avian Disease, College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China;3.College of Animal Science and Veterinary Medicine, Inner Mongolian Agricultural University, Huhhot 010018, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

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Abstract: In this study, pig IL-2 eukaryotic expression plasmid pcDNA-IL-2 was constructed and its adjuvant effect was investigated. Four groups of pigs were immunized with sterilized water, empty vector pcDNA3.1(+), PRRSV-SC2-OFR5 gene eukaryotic expression plasmid pcDNA-PRRSV-SC2-ORF5 and a mixed solution of pcDNA-IL-2 and pcDNA-PRRSV-SC2-ORF5 respectively. Serum of pig in each group was collected at different time post inoculation, and antibody to PRRSV was measured by ELISA. Peripheral T lymphocytes were acquired from a small quantity of blood of each pig, and the activity of lymphocyte proliferation was detected by MTT methods after being cultured with RPMI1640 culture medium. Content of CD+4and CD+8 of peripheral T lymphocytes in each groups of pigs were tested by cytospectrometry. Results showed that the antibody and peripheral T lymphocytes level, content of CD+4,CD+8 cell of the pigs immunized with both pcDNA-IL-2 and pcDNA-PRRSV-SC2-ORF5 were significantly higher than that of the pigs immunized with pcDNA-PRRSV-SC2-ORF5 alone, indicate that plasmid pcDNA-IL-2 can be used as a adjuvant to enhance PRRS gene vaccine.