ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (10): 1093-1097.doi:

• 预防兽医 • Previous Articles     Next Articles

Purification of Merozoites of Theileria spp. from Infected Erythrocytes in Sheep Using Hemolysin

LI You-quan;LUO Jian-xun;GUAN Gui-quan;MA Mi-ling;GAO Jin-liang;LIU Zhi-jie;
DANG Zhi-sheng;LIU Ai-hong;LIU Jun-long;REN Qiao-yun;LU Bing-yi;BAI Qi;YIN Hong   

  1. Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences，Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

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Abstract: A simple and rapid method for purifying merozoites of Theileria spp. from infected erythrocytes in sheep or goats was developed. Infected erythrocytes were effectively lysed using hemolysin Ah-1 produced by Aeromonas hydrophila, and the lysate was subjected to centrifugation in a Percoll discontinuous density gradient at common speed. Light microscopical study showed that the merozoites purified retained their intact basic structure and were free of infected erythrocyte membrane and other host-cell components