ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (10): 1088-1092.doi:

• 预防兽医 • Previous Articles     Next Articles

Detection of SulfonamidesResistant Genes of Pathogenic Salmonella and Ecoli by Duplex PCR

YANG Yun-fei;WANG Hong-ning;TAN Xue-mei;ZHANG An-yun;YANG Xin;XIA Qing-qing;ZENG Bo   

  1. 1College of Animal Science and Technology，Sichuan Agricultural University，Yaan 625014，China;2Sichuan University “985” Project, College of Life Science, Sichuan University，Chengdu 610064, China; 3The Station of Supervising Animal Epidemic Prevention of Chengdu, Chengdu 610014,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

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Abstract: The Sulfonamides-resistant genes Sul1 ，Sul2， Sul3 were detected by duplex PCR from 33 Salmonella strains and 103 E.coli strains from pig and chicken The results showed that Sul1,Sul2 genes of 17 Salmonella strains were detected from 19 SD resistant Salmonella strains demonstrated by MIC, and the detection rate was 89.47%. The coincidence rate between duplex PCR assays and MIC assays was 89.47%. Sul1 ，Sul2 genes of 53 E.coli strains were detected from 56 SD resistant E.coli strains. The rate of coincidence of duplex PCR assays and MIC assays was 94.64%.The rate of coincidence between duplex PCR assays and common PCR assays is 98.5%. It was only taken 7 hours to complete the whole duplex PCR test.