Abstract: According to the published genome sequences of infectious bronchitis virus (IBV） on GenBank, nineteen pairs of primers were designed and the genome of isolated SAIBk strain of IBV was amplified by RTPCR. All the RT-PCR products were cloned into the pMD18-T vector and sequenced. The genome sequence of SAIBk strain was 27 520 bp in length. The ratio of A+T in the genome was 61.74%. Compared with the genome of Beaudette, Mass41, Cal99, BJ and KQ6, the similarity was 87.2%, 87.6%, 87.2%, 85.6% and 87.5%, respectively. The 3′UTR and 5′UTR were the most conservative region in the genome, the similarity was between 90.9%-97.7%. The S1 gene have the most variations, the similarity was between 74.8%-83.2%.The phylogenetic analysis results showed that the SAIBk genome have a close relationship with S14,LX4,BJ strains which were isolated in China.