ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (1): 66-71.doi:

• 预防兽医 • Previous Articles     Next Articles

Cloning and Analysis of a 8.5 kb Sequence from the 3′ End of Transmissible Gastroenteritis Virus Genome

  

  1. 1Animal Biotechnology Centre, Sichuan Agricultural University ,Yaan 625014,China;2Dongguan EntryExit Inspection And Quarantine Bureau,Dongguan 523072,China; 3 Animal Science and Techology College, Sichuan Agricultural University,Yaan 625014,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-01-25 Published:2007-01-25

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Abstract: Six fragments about 1.5 kb,2.0 kb,1.1 kb,1.4 kb,1.4 kb,1.3 kb of transmissible gastroenteritis virus strain SCY were amplified by PCR , then were cloned into the pGMT vector and sequences were determined in Takara . 8 495 bp sequence was obtained after assembling by CAP program.Bioinformatics analysis showed that the 8 495 bp sequence included S,M,N,E,NSP3a,NSP3b,ORF7 genes. Phylogenetic trees based on S,M,N genes showed that TGEV SCY strain was closed to the TS,HN2002,TO14,pudue strains.TGEV SCY didn’t bias remarkably on any codons,but some of the amino acids biased slightly on the codons which ended by A or T.