ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (7): 722-726.doi:

• 研究简报 • Previous Articles     Next Articles

New Allele of BoLA-DRB3 Gene against Disease in Bovine

WANG Xing-ping;XU Shang-zhong;ZAN Lin-sen;GAO Xue;REN Hong-yan;CHEN Jin-bao   

  1. 1.Animal Science-Technology College of Northwest A & F University, Yangling 712100, China; 2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-07-25 Published:2006-07-25

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Abstract: BoLA-DRB3 is a member of the MHC (Major Histocompatibility Complex), which plays a central role in immune system and disease resistance of bovine. The exon2 of BoLA-DRB3 gene is a mainly functional region, which codes the antigen and participates in the body’s immune response. In this study, the 307 bp fragment including the exon2 in Luxi , Qinchuan, Nanyang and Jinnan cattle was amplified by polymerase chain reaction, which was digested with restriction endonuclease MspⅠ. The results showed new allele was produced by the mutation of C to A at position 154 in exon2. Statistical results of χ2 test indicated that genetic polymorphism sites of the exon2 of BoLA-DRB3 locus in Luxi cattle did not fit Hardy-Weinberg equilibrium (P<0.05).