Abstract: The genetic variation of Adipocyte fatty acid binding protein gene on the sequence sites of 85 and 1 805 in 96 chicken including Youxi Partridge, Luyuan and Recessive Whitefeather chicken were detected by PCR-RFLP and DNA sequence analysis. The results showed as follows: 1）All these chicken breeds had genetic variation in A-FABP gene on sites 85 and 1 805. Recessive Whitefeather chicken presented mostly Cc genotype while other two breeds presented mostly cc genotype. The allele c had high frequency in three chicken breeds and in Youxi partridge chicken was the highest and Recessive Whitefeather chicken was the lowest. 2）The genetic variation on 85 site had no effect on amino acid sequence of A-FABP and 1 805 site had effect on amino acid sequence of A-FABP: Pro→Ser which suggested that the chicken quality may have relation to A-FABP’s expression possibly controlled by genetic variation.The research built the base for further analysis on relation between genetic variation of A-FABP and the IMF quantity, and molecular marker’s application in breeding.