ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (2): 116-120.doi:

• 研究报告 • Previous Articles     Next Articles

Designing and Screening of PCR Primers for Sexing Bovine Pre-implantation Embryos

WANG Zong-li;WANG Dong;CHENG Jin-hua; YANG Bo;ZHU Hua-bin; HAO Hai-sheng; GUO Yi
  

  1. 1.College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070，China；2.Institute of Animal Science ,Chinese Academy of Agricultural Sciences, Beijing 100094，China；3.Institute of Zoology, Chinese Academy of Sciences, Beijing 161006 ,China;4.Grassland Research Institute of Chinese Academy of Agricultural Sciences,Huhhot 010010,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-02-25 Published:2005-02-25

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Abstract: 5 pairs of bovine male-specific PCR primers were designed based on the sequences of bovine Y-specific repeat sequence, testis-specific protein gene on Y chromosome and sex determining region Y gene (SRY) , meanwhile, based on skeletal alpha action precursor gene and bovine 1.715 satellite DNA, 4 pairs of bovine-specific PCR primers were designed as internal control primers. After PCR amplification of bovine genomic DNA with different primers, 4 pairs of bovine male-specific primers and 1 pair of bovine-specific primers could work very well. Two primer combinations of bovine male-specific PCR primers and internal control primers, B34/A12 and B78/A12, were obtained for sexing bovine preimplantation embryo after multiplex PCR amplifications of bovine genomic DNA, fibroblasts, and clone embryos.