单细胞转录组测序技术在家禽中的研究进展与发展趋势

doi:10.11843/j.issn.0366-6964.2025.12.001

Abstract

Abstract: Single-cell RNA sequencing (scRNA-seq) is revolutionizing poultry biology research by resolving cellular heterogeneity and dynamic gene expression at single-cell resolution. This review systematically summarizes the advances in scRNA-seq applications for poultry: the technology enables cell-by-cell analysis, offering novel strategies to enhance egg production efficiency, improve meat quality, and boost disease resistance. However, current research faces bottlenecks including incomplete cellular atlas coverage, scarce functional validation systems, and high agricultural conversion costs. Future efforts should prioritize developing low-cost sequencing, integrating multi-omics data (e.g., scATAC-seq and metabolomics), and implementing targeted validation via CRISPR/Cas9-mediated avian models. These approaches will drive intelligent design of precision breeding and reproductive regulation. This review provides theoretical foundations and technical prospects for deciphering cell fate determination mechanisms in poultry and their practical applications.

Key words: single-cell RNA sequencing, avian follicular development, egg-laying traits, poultry breeding

CLC Number:

S813

DONG Tianshu, KE Wentao, LIANG Hao, LI Shaoming, LI Peng, WANG Jiaxiang, WU Yan, PI Jinsong, XIE Yang, LUAN Yan. Research Advances and Future Perspectives of Single-Cell RNA Sequencing Technology in Poultry[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 5943-5951.

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Research Advances and Future Perspectives of Single-Cell RNA Sequencing Technology in Poultry

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