Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (7): 3423-3432.doi: 10.11843/j.issn.0366-6964.2025.07.034

• Preventive Veterinary Medicine • Previous Articles     Next Articles

The Inhibitory Effect of Transcription Factor CEBPB on the Replication of Porcine Deltacoronavirus in vitro

CHEN Zhuoqi1,2(), FAN Liyuan2, ZHONG Chunyan5, YU Yanfei2, LI Jizong2,3,4,*(), LI Bin2,3,4,*(), YUAN Xiaomin1,*()   

  1. 1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
    2. Key Laboratory of Engineering Technology for Animal Biological Products, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
    3. College of Life Science, Jiangsu University, Zhenjiang 212013, China
    4. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
    5. Department of Bioengineering, Southwest Guizhou Vocational and Technical College for Nationalities, Xingyi, 562400, China
  • Received:2024-07-05 Online:2025-07-23 Published:2025-07-25
  • Contact: LI Jizong, LI Bin, YUAN Xiaomin E-mail:475846734@qq.com;lijizong22@sina.com;libinana@126.com;yxm1230@hunau.edu.cn

Abstract:

This study aims to explore the impact of CCAAT/enhancer-binding protein beta (CEBPB) on porcine deltacoronavirus (PDCoV) replication. PDCoV was inoculated in LLC-PK1 cells, and the expression levels of CEBPB at different time points were detected. Overexpression and knockdown of CEBPB were performed, and their effects on PDCoV replication were assessed using qPCR and Western blot. Furthermore, the role of CEBPB in the PDCoV replication cycle was clarified. Viral proteins that influenced the upregulation of CEBPB were screened using qPCR, and the colocalization of PDCoV viral proteins with CEBPB was observed using confocal microscopy. The results showed that CEBPB expression increased with prolonged virus infection time. Overexpression of CEBPB significantly inhibited PDCoV replication, while knockdown of CEBPB promoted virus replication. CEBPB mainly acted during the replication stage of PDCoV, and PDCoV Nsp4 could promote CEBPB expression. Confocal microscopy revealed colocalization of Nsp4 and CEBPB. In conclusion, CEBPB could significantly inhibit PDCoV replication in vitro, which provided important data for the development of antiviral drugs based on CEBPB.

Key words: CEBPB, PDCoV, Nsp4, anti-virus

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