Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (5): 2146-2153.doi: 10.11843/j.issn.0366-6964.2024.05.032

• PREVENTIVE VETERINARY MEDICINE • Previous Articles     Next Articles

Study on Purification of Bovine Brucellosis by Competitive ELISA and Indirect ELISA

ZHAO Canqi1,2, FENG Yu1, LÜ Lang1, LI Yanjun3, WEI Yulei4, DING Jiabo1, CHEN Xiang5, JIANG Hui1*   

  1. 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Animal Disease Prevention and Control Center of Dali Prefecture, Yunnan Province, Dali 671005, China;
    3. Animal Disease Prevention and Control Center of Alxa Youqi, Inner Mongolia, Alxa Youqi 737300, China;
    4. Comprehensive Security and Technology Promotion Center in Zongbieli Town, Alxa Zuoqi, Inner Mongolia, Alxa Zuoqi 753421, China;
    5. Jiangsu University's Collaborative Innovation Center for the Prevention and Control of Important Animal Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou 225000, China
  • Received:2023-07-11 Online:2024-05-23 Published:2024-05-27

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Abstract: The aim of this study was to evaluate the efficacy of the combination of competitive ELISA and indirect ELISA methods in the purification of brucellosis, hoping to provide technical support for the prevention and control of brucellosis. A total of 3 271 bovine serum samples from a cattle farm in the northwest of China were tested using the competitive ELISA (cELISA) antibody detection kit, the indirect ELISA (iELISA) antibody detection kit, and the micro-complement fixation test (mCFT) which developed by the writers team. This study adopted a purification strategy of cELISA for preliminary screening, iELISA for confirm diagnosis. The positive cattle were eliminating, the suspect cattle and negative cattle were respectively quarantined after disinfecting completely. After the previous detection, the group was sampled every month for multiple “Detection-Elimination” cycles. When the individual positive rate was below 2% or all negative, the results were verified using a micro-complement fixation test (mCFT). The test were carried on for 2 months after all the bovine turned negative to ensure the successful of the purification. The results showed that, after the implementation of this purification strategy, the positive rate of the infected group with a positive rate of 35.36% decreased to 25.41% in the first month, 7.16% in the second month, and 1.86% in the third month. By the fourth month, the group had achieved a comprehensive negative conversion, and the negative rate by mCFT verification was 100%. The detection continued for 2 months, and the individual positive rate was 0. So far, the brucellosis purification of the infected group was realized, making nearly half of the cattle in the group free from culling, greatly reducing the economic loss. In conclusion, cELISA is suitable for the preliminary screening, while iELISA is suitable for confirmed diagnosis of brucellosis. The combination of two methods, through multiple consecutive tests, can achieve a comprehensive purification of brucellosis infected within a relatively short period of time.

Key words: brucellosis, competitive ELISA, indirect ELISA, micro-complement fixation test, preliminary screening, confirmed diagnose

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