Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (4): 1370-1380.doi: 10.11843/j.issn.0366-6964.2024.04.003

• REVIEW • Previous Articles     Next Articles

Advances in Animals Sperm Sexing Techniques

CHEN Hongyu1, WEI Yating1, LI Ruoxi2, GAO Liutao2*, LIU Shenhe1*   

  1. 1. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China;
    2. Henan Dingyuan Cattle Breeding Co. LTD., Zhengzhou 450000, China
  • Received:2023-10-10 Online:2024-04-23 Published:2024-04-26

Abstract: Sex control is a technique in which a female animal produces offspring with a specific sex through human intervention. Since the separation of X sperm and Y sperm is the technical basis for the realization of sperm sexing in animals, this paper summarizes and compares the new techniques for the separation of sperm before fertilization, including flow cytometry, immunological approach of sperm sexing, pH separation, microfluidics and nanotechnology. The activation of TLR7/8 signaling pathway by R848 (Resiquimod) in immunological isolation is a potential method for sperm isolation, aiming to provide a theoretical basis for sperm sexing techniques.

Key words: sperm sexing, X sperm, Y sperm, immunological approach of sperm sexing, TLR7/8

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