宁乡猪FABPs、SLC13A5和NR1H4基因多态性及其与IMF含量的关联分析

doi:10.11843/j.issn.0366-6964.2022.11.003

Abstract

Abstract: The study aimed to explore the polymorphisms of FABPs, SLC13A5 and NR1H4 genes in Ningxiang pigs and their association with intramuscular fat (IMF) content. In the experiment, the polymorphisms of FABPs, SLC13A5 and NR1H4 genes in 172 Ningxiang barrows were detected using PCR-RFLP and Sanger sequencing, and the association between them and IMF content was explored. The results showed that the Hinf Ⅰ digestion site of H-FABP gene was polymorphic in Ningxiang pigs, and the IMF content of individuals with Hh genotype was the highest, which was significantly different from that of individuals with HH and hh genotypes (P<0.01). Only DD and Dd genotypes were detected in H-FABP using Hae Ⅲ enzyme, and the IMF content of individuals with Dd genotype was significantly higher than that of individuals with DD genotype (P<0.01). There were 3 haplotypes and 5 haplotype combinations in the analysis of the Hinf Ⅰ and Hae Ⅲ sites of H-FABP gene, and the pigs with haplotype combination HD/hd had the best IMF content. The results of H-FABP Msp I digestion and A-FABP sequencing showed that there was no polymorphism at the detected sites in Ningxiang pigs. No significant difference was observed in IMF content among different genotypes at the polymorphism site of SLC13A5 (g.50705299T/C) and NR1H4 (g.83607915G/A) gene (P>0.05). In conclusion, the polymorphisms of H-FABP Hinf Ⅰ and Hae Ⅲ sites were significantly associated with IMF content in Ningxiang pigs, and the pigs with haplotype combination HD/hd had the highest IMF content. The above results could provide reference for the early molecular breeding of IMF content trait in Ningxiang pigs.

Key words: Ningxiang pigs, FABPs, SLC13A5, NR1H4, IMF

CLC Number:

S828.2

LI Yiyang, YIN Shishu, LIAO Yinchang, XU Kang, ZHANG Yuebo, HE Jun. Polymorphisms of FABPs, SLC13A5 and NR1H4 Genes in Ningxiang Pigs and Their Association with IMF Content[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3737-3747.

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Polymorphisms of FABPs, SLC13A5 and NR1H4 Genes in Ningxiang Pigs and Their Association with IMF Content

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