牛传染性鼻气管炎病毒诱导MDBK细胞线粒体损伤模型的建立

doi:10.11843/j.issn.0366-6964.2022.09.027

Abstract

Abstract: The purpose of this study was to investigate the optimal time and concentration of bovine infectious rhinotracheitis virus (IBRV)-induced mitochondrial damage in MDBK cells. 0.5, 1.0, 1.5 and 2.0 MOI IBRV were used to infect MDBK cells for 6, 12, 24, 36 and 48 h. Electron microscopy and flow cytometry were used to detect the MMP and abnormal opening of MPTP. The results showed that when MDBK cells were infected with different MOI IBRV for 12 h, the MPTP decreased significantly in the Infection group compared with the control group, and decreased with the increase of exposure dose except 2.0 MOI;The MMP started to decrease significantly after 1.5 MOI; When the MDBK cells were infected with 1.5 MOI IBRV for 6, 12, 24 and 36 h respectively, compared with the control group, the MPTP and MMP in the Infection group were significantly decreased from 6 h, and decreased with the prolongation of the virus infection;The results of transmission electron microscopy showed that MDBK cells infected with IBRV could cause mitochondrial damage and partial vacuolization after 6 h, complete vacuolization of mitochondria after 24 h, and virus particles could be seen around mitochondria. The results show that IBRV infection can induce mitochondrial damage in MDBK cells, and infecting MDBK cells at 1.5 MOI for 6-24 h is the best time and concentration to induce cell mitochondrial damage. This time and concentration can be used for IBRV-related drug research, and provide a theoretical basis for further research on mitochondrial damage.

Key words: IBRV, mitochondrial damage, MMP, MPTP

CLC Number:

S852.659.1

GUO Xueping, MA Yingcai, LI Zelong, WANG Tianzi, GAO Hanya, XU-Li Yina, WU Yufeng, ZHONG Qi, YAO Gang, MA Xuelian. Establishment of a Model of Mitochondrial Damage Induced by Bovine Infectious Rhinotracheitis Virus in MDBK Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3132-3139.

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Establishment of a Model of Mitochondrial Damage Induced by Bovine Infectious Rhinotracheitis Virus in MDBK Cells

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