卵黄抗体对鸽毛滴虫与白色念珠菌感染的治疗效果

doi:10.11843/j.issn.0366-6964.2022.02.033

Acta Veterinaria et Zootechnica Sinica ›› 2022, Vol. 53 ›› Issue (2): 658-662.doi: 10.11843/j.issn.0366-6964.2022.02.033

• RESEARCH NOTES • Previous Articles

The Therapeutic Effect of Yolk Antibody on the Infection of Trichomonas gallinae and Candida albicans

WANG Fei¹, ZHANG He¹, CAI Yuhan¹, HUANG Zhongzuo¹, YAN Peijie¹, WANG Lige¹, SUN Hong³, LI Fuhuang^2*, HE Cheng^1*

1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
2. Beijing Animal Husbandry Station, Beijing 100107, China;
3. Beijing Youdi Pigeon Industry Co., Ltd., Beijing 100188, China

Received:2021-05-07 Online:2022-02-23 Published:2022-03-02

Abstract

Abstract: This study aimed to prepare the dual yolk antibody of Trichomonas gallinae and Candida albicans and study its therapeutic effect. Based on isolates of T. gallinae and C. albicans, we prepared a bivalent vaccine, harvested high-level antibody by inoculating to layers and produced into powders using freeze-dry system under low temperature. Moreover, antibody levels were determined to check quality control using in-house ELISA method. To test efficacy, pigeon models were developed by artificial infection with T. gallinae and C. albicans, respectively. The OD values of T. gallinae antibody and C. albicans antibody in the yolk reached their peaks after second immunization, which were 0.90 and 0.66, respectively, and the antibody maintenance time reached 2 months.In vivo study, both oral- and crop lesions score were reduced significantly(P<0.05)post feeding with 1 g of hyperimmune antibody powders per bird for 5 days and no significant difference was found compared to those pigeons treated with metronidazole and nystatin. Moreover, pathogen loads were reduced greatly compared to the control group(P<0.01). The hyperimmune antibody powder is a promising approach to replace metronidazole and nystatin, contributing to a novel solution for healthy development of pigeon industry.

Key words: meat pigeon, Trichomonas gallinae, Candida albicans, hyperimmune antibody powder, metronidazole, nystatin

CLC Number:

S858.395

WANG Fei, ZHANG He, CAI Yuhan, HUANG Zhongzuo, YAN Peijie, WANG Lige, SUN Hong, LI Fuhuang, HE Cheng. The Therapeutic Effect of Yolk Antibody on the Infection of Trichomonas gallinae and Candida albicans[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 658-662.

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The Therapeutic Effect of Yolk Antibody on the Infection of Trichomonas gallinae and Candida albicans

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