民猪脂肪细胞分化过程中抗寒相关基因表达规律研究

doi:10.11843/j.issn.0366-6964.2021.07.026

Abstract

Abstract: This study aimed to investigate the expression of thermogenic genes and beige fat marker genes during the differentiation of Min pig adipocytes and to provide a basis for further study on the molecular mechanism of differentiation of Min pig adipocytes into beige adipocytes. Adipose tissue of 1-month-old Min pig was collected, preadipocytes were isolated, cultured and induced to adipogenesis, cell morphology was observed during differentiation and identified by Oil red O staining; The expression levels of thermogenic genes UCP3, PGC-1α, PPARα, and beige fat marker genes EBF2, CD81, PDGFRα were detected on day 0, 2, 4, 6 and 8 after differentiation. The results showed that the number of lipid droplets increased with the time of differentiation increased. The result of Oil red O staining showed that most of the cells reached the stage of mature adipocytes. The expression levels of PGC-1α, EBF2 and PDGFRα were significantly increased on day 2 compared with those on day 0 (P<0.01) and reached the highest level, and decreased on day 4, 6, and 8. The expression of UCP3 was significantly increased at day 4, 6, and 8(P<0.01), and decreased at day 8. The expression levels of PPARα and CD81 were significantly increased on day 2 (P<0.01), and there was no significant change on day 4, 6 compared with day 2. In conclusion, this study successfully conducted adipogenic differentiation of Min pig preadipocytes, revealed the expression of thermogenic and beige fat marker genes during differentiation, and laid a foundation for further study on the molecular mechanism of differentiation of Min pig preadipocytes into beige adipocytes and cold-resistance of Min pig.

Key words: Min pig, beige fat, thermogenic gene, beige fat marker gene

CLC Number:

S828.2

FAN Wenbo, MA Hong, MA Shouzheng, WANG Liang, LIU Di. Expression of Cold Resistance Related Genes during Differentiation of Adipocytes in Min Pig[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2044-2051.

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Expression of Cold Resistance Related Genes during Differentiation of Adipocytes in Min Pig

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