牛瘤胃上皮细胞中挥发性脂肪酸吸收相关蛋白在白细胞介素10诱导下的差异表达研究

doi:10.11843/j.issn.0366-6964.2021.03.015

Abstract

Abstract: This study aimed to investigate the effect of inflammatory factor (interleukin-10, IL-10) on the expression of genes related to volatile fatty acid (VFA) absorption in bovine rumen epithelial cells. The experiment adopted a randomized block design. Under the conditions of in vitro culture of rumen epithelial cells, the effect of 50 ng·mL^-1 IL-10 on genes expression of nuclear factor (NF-κB), putative anion transporter 1 (PAT1), anion exchanger 2(AE2), monocarboxylate transporter 1(MCT1), monocarboxylate transporter 4 (MCT4), Na^-/H⁺exchanger 1 (NHE1) in bovine rumen epithelial cells was detected. The results showed that, under neutral conditions, IL-10 addition significantly down-regulated the expression of NF-κB, PAT1, MCT1, NHE1 genes (P<0.05), and significantly up-regulated the expression of AE2 gene (P<0.05), while had no significant effect on the gene expression of MCT4. Under acidic conditions, the expression of NF-κB gene in the IL-10 addition group was significantly inhibited (P<0.05), while the expression of PAT1, MCT1, and MCT4 genes were significantly promoted (P<0.05). Compared with the condition of pH7.2 group, the NF-κB gene expression in the pH5.5 group was significantly increased (P<0.05). The results suggest that, under inflammatory conditions, interleukin-10 as an anti-inflammatory factor can alleviate the inflammatory response of rumen epithelial cells and promote the genes expression of VFA absorption-related protein.

Key words: IL-10, rumen epithelial cells, carrier protein, volatile fatty acids

CLC Number:

S823.2

HOU Hongyan, WANG Zhonghao, HUO Wenjie, LIU Qiang, ZHANG Jing, CHEN Lei, PEI Caixia, GUO Gang. Differential Expression of Volatile Fatty Acid Absorption Related Proteins in Bovine Rumen Epithelial Cells Induced by Interleukin 10[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 714-722.

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Differential Expression of Volatile Fatty Acid Absorption Related Proteins in Bovine Rumen Epithelial Cells Induced by Interleukin 10

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