miR-186-5p与α-MSH互作对绵羊黑素细胞黑色素生成的影响

doi:10.11843/j.issn.0366-6964.2021.003

Abstract

Abstract: Melanogenesis is regulated by many genes and miRNAs. This study aimed to explore the effect of interaction between miR-186-5p and α-MSH on melanogenesis in sheep melanocytes. The miR-186-5p expression vector was transfected into sheep melanocytes and α-MSH was also added to the cells at the same time. Expression patterns of associated genes(MITF, TYR, TYRP1, TYRP2) were detected by qRT-PCR and Western blotting. The expression and localization of MITF protein in sheep melanocytes were further detected by immunohistochemistry. The melanin contents and the migration capability of melanocytes were measured by spectrophotometry and scratch test, respectively. Three experiment groups were set up, containing miR-186-5p transfected group, miR-186-5p+α-MSH interaction group and negative control group, and there were 3 replicates in each group. The results showed that the expression of MITF, TYR, TYRP1 and TYRP2 were down-regulated, the production of melanin was inhibited in miR-186-5p transfected group, while the above effects of miR-186-5p were alleviated by adding α-MSH to the cells. In addition, miR-186-5p also could inhibit cell division and block cell migration, but α-MSH could not affect the negative effects of miR-186-5p. In summary, the results indicated that in sheep melanocytes, both miR-186-5p and α-MSH were involved in regulating melanogenesis.Between them, miR-186-5p mainly played a negative regulatory role, while α-MSH was involved in the positive regulation of related genes and relieved the inhibitory effect of miR-186-5p. It was worth noting that miR-186-5p played an important role in regulating cell proliferation and migration, while α-MSH did not participate in the related regulation.

Key words: miR-186-5p, sheep melanocytes, a-MSH, TYR gene family, melanogenesis

CLC Number:

S826.2

XU Dongmei, ZHU Zhiwei, TANG Zhongwei, LI Pengfei, DONG Changsheng, ZHAO Yujun. Effect of Interaction between miR-186-5p and α-MSH on Melanogenesis in Sheep Melanocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 19-27.

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Effect of Interaction between miR-186-5p and α-MSH on Melanogenesis in Sheep Melanocytes

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