叶酸补饲对隐性乳房炎奶牛免疫力及产奶性状相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2020.11.012

Abstract

Abstract: The study aimed to explore the effect and related molecular mechanisms of folic acid supplementation on the immunity and milking related traits of dairy cattle with subclinical mastitis, which would lay a foundation for folic acid supplementation of cows with subclinical mastitis. A total of 29 lactating cows were selected based on their parity, body weight and somatic cell count (SCC>500×10³ cells·mL^-1 for 2 consecutive months) from a Holstein cow population in a farm near Beijing, China. The 29 cows were randomly divided into feeding group (n=18) and control group (n=11). The 18 cattle in feeding group were fed with extra-coated folic acid (120 mg/500 kg per day) for two weeks, and no treatment in the control group. Then collected DHI data (including somatic cell count, daily milk yield, etc.) were analyzed. Subsequently, four individuals were randomly selected in each group to collect leukocytes, extract RNA and perform transcriptome sequencing. DESeq2 was used to perform differential expression gene analysis using RNA-Seq data. KOBAS3.0 pathway database was used for KEGG and GO functional analysis. Somatic cell counts (SCC) showed a significant decrease (P<0.05) after folic acid supplementation, however, no significant effect was observed on milking related traits. A total of 277 differentially expressed genes (P<0.05, log₂|Fold Change|>1) were detected in the comparison between the two groups, including 105 up-regulated and 172 down-regulated genes. GO analysis showed that these genes were mainly enriched in the immune-related functions terms， including inflammation, immune response, regulation of cytokine production, cell differentiation, leukocyte chemotaxis and migration, and regulation of immune system processes. In addition, KEGG pathway analysis revealed that these DEGs were involved in innate immunity and cellular immune response pathways. In conclusion, folic acid supplementation has an important impact on immune-related traits and expression level of genes related to immune pathways. The current research provides a foundation for the promotion and application of folic acid supplementation.

Key words: Chinese Holstein cows, folic acid supplementation, subclinical mastitis, immune-related traits, milking traits

CLC Number:

S823.91

LIU Xueqin, WANG Di, MI Siyuan, ZHANG Ruiqiang, SHI Liangyu, YU Ying. The Effect of Folic Acid Supplementation on the Expression of Genes Related to Immunity and Milking Traits in Subclinical Mastitis Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2731-2742.

References 43

[1]	GUÉANT J L,NAMOUR F,GUÉANT-RODRIGUEZ R M,et al.Folate and fetal programming:a play in epigenomics?[J].Trends Endocrinol Metab,2013,24(6):279-289.
[2]	CAWLEY S,MULLANEY L,MCKEATING A,et al.A review of European guidelines on periconceptional folic acid supplementation[J].Eur J Clin Nutr,2016,70(2):143-154.
[3]	LY A,ISHIGURO L,KIM D,et al.Maternal folic acid supplementation modulates DNA methylation and gene expression in the rat offspring in a gestation period-dependent and organ-specific manner[J].J Nutr Biochem,2016,33:103-110.
[4]	WANG Y,JIN Y,WANG Y,et al.The effect of folic acid in patients with cardiovascular disease:A systematic review and meta-analysis[J].Medicine (Baltimore),2019,98(37):e17095.
[5]	SAHAKYAN V,DUELEN R,TAM W L,et al.Folic acid exposure rescues spina bifida aperta phenotypes in human induced pluripotent stem cell model[J].Sci Rep,2018,8(1):2942.
[6]	YADAV M K,MANOLI N M,MADHUNAPANTULA S R V.Comparative assessment of vitamin-b12,folic acid and homocysteine levels in relation to p53 expression in megaloblastic anemia[J].PLoS One,2016,11(10):e164559.
[7]	REYNOLDS E H.The neurology of folic acid deficiency[J].Handb Clin Neurol,2014,120:927-943.
[8]	GOMES S,LOPES C,PINTO E.Folate and folic acid in the periconceptional period:recommendations from official health organizations in thirty-six countries worldwide and WHO[J].Public Health Nutr,2016,19(1):176-189.
[9]	TOIVONEN K I,LACROIX E,FLYNN M,et al.Folic acid supplementation during the preconception period:A systematic review and meta-analysis[J].Prev Med,2018,114:1-17.
[10]	ZHENG J S,GUAN Y H,ZHAO Y M,et al.Pre-conceptional intake of folic acid supplements is inversely associated with risk of preterm birth and small-for-gestational-age birth:a prospective cohort study[J].Br J Nutr,2016,115(3):509-516.
[11]	WANG S P, YIN Y L,YIN Q,et al.Effects of folic acid on the performance of suckling piglets and sows during lactation[J].J Sci Food Agric,2011,91(13):2371-2377.
[12]	MUNYAKA P M,TACTACAN G,JING M,et al.Immunomodulation in young laying hens by dietary folic acid and acute immune responses after challenge with Escherichia coli lipopolysaccharide[J].Poult Sci,2012,91(10):2454-2463.
[13]	JANIST N,SRICHANA P,ASAWAKARN T,et al.Effect of supplementing the laying hen diets with choline,folic acid,and vitamin B12 on production performance,egg quality,and yolk phospholipid[J].Livest Sci,2019,223:24-31.
[14]	NOURI S,GHALEHKANDI J G,HASSANPOUR S,et al.Effect of in ovo feeding of folic acid on subsequent growth performance and blood constituents levels in broilers[J].Int J Pept Res Ther,2018,24(3):463-470.
[15]	BAGHERI S,JANMOHAMMADI H,MALEKI R,et al.Laying hen performance,egg quality improved and yolk 5-methyltetrahydrofolate content increased by dietary supplementation of folic acid[J].Anim Nutr,2019,5(2):130-133.
[16]	SCHWAB E C,SCHWAB C G,SHAVER R D,et al.Dietary forage and nonfiber carbohydrate contents influence B-vitamin intake,duodenal flow,and apparent ruminal synthesis in lactating dairy cows[J].J Dairy Sci,2006,89(1):174-187.
[17]	GONÇALVES J L,KAMPHUIS C,MARTINS C M M R,et al.Bovine subclinical mastitis reduces milk yield and economic return[J].Livest Sci,2018,210:25-32.
[18]	马裴裴,俞英,张沅,等.中国荷斯坦牛SCC变化规律及其与产奶性状之间的关系[J].畜牧兽医学报,2010, 41(12):1529-1535.MA P P,YU Y,ZHANG Y,et al.The distribution of SCC and its correlation with milk production traits in Chinese Holsteins[J].Acta Veterinaria et Zootechnica Sinica,2010,41(12):1529-1535.(in Chinese)
[19]	BOBBO T,RUEGG P L,STOCCO G,et al.Associations between pathogen-specific cases of subclinical mastitis and milk yield,quality,protein composition,and cheese-making traits in dairy cows[J].J Dairy Sci,2017,100(6):4868-4883.
[20]	张志超,陈思倩,刘刚,等.围产期奶牛饲喂包被叶酸对早期产奶性能的影响[J].中国奶牛,2019(5):11-14.ZHANG Z C,CHEN S Q,LIU G,et al.Effects of feeding folic acid on the early milk production performance of cows during perinatal period[J].China Dairy Cattle,2019(5):11-14.(in Chinese)
[21]	KHAN M Z,ZHANG Z C,LIU L,et al.Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows[J].Asian-Australas J Anim Sci,2020,33(9):1507-1519.
[22]	宋敏艳.奶牛金葡菌乳房炎抗性的全基因组DNA甲基化表达调控及候选基因功能验证[D].北京:中国农业大学,2017.SONG M Y.The regulation of Genome-wide DNA methylation and functional verification of candidate genes for bovine Staphylococcus aureus mastitis resistance[D].Beijing:China Agricultural University,2017.(in Chinese)
[23]	张志超.包被叶酸提高围产期荷斯坦牛免疫力的分子基础研究[D].北京:中国农业大学,2016.ZHANG Z C.Study on molecular mechanisms of folic acid to improve the immunity of transition Holstein cow[D].Beijing:China Agricultural University,2016.(in Chinese)
[24]	BAE D,CHON J W,KIM D H,et al.Effect of folic acid supplementation on proliferation and apoptosis in bovine mammary epithelial (MAC-T) cells[J].Anim Biotechnol,2020:1-9.
[25]	GRAULET B,MATTE J J,DESROCHERS A,et al.Effects of dietary supplements of folic acid and vitamin B12 on metabolism of dairy cows in early lactation[J].J Dairy Sci,2007,90(7):3442-3455.
[26]	LI H Q,LIU Q,WANG C,et al.Effects of dietary supplements of rumen-protected folic acid on lactation performance,energy balance,blood parameters and reproductive performance in dairy cows[J].Anim Feed Sci Technol,2016,213:55-63.
[27]	PREYNAT A,LAPIERRE H,THIVIERGE M C,et al.Effects of supplementary folic acid and vitamin B12 on hepatic metabolism of dairy cows according to methionine supply[J].J Dairy Sci,2010,93(5):2130-2142.
[28]	GIRARD C L,LAPIERRE H,MATTE J J,et al.Effects of dietary supplements of folic acid and rumen-protected methionine on lactational performance and folate metabolism of dairy cows[J].J Dairy Sci,2005,88(2):660-670.
[29]	SRIKANDAKUMAR A,JOHNSON E H.Effect of heat stress on milk production,rectal temperature,respiratory rate and blood chemistry in holstein,jersey and australian milking zebu cows[J].Trop Anim Health Prod,2004,36(7):685-692.
[30]	WANG C,LIU Q,GUO G,et al.Effects of rumen-protected folic acid and branched-chain volatile fatty acids supplementation on lactation performance,ruminal fermentation,nutrient digestion and blood metabolites in dairy cows[J].Anim Feed Sci Technol,2019,247:157-165.
[31]	DU H S,WANG C,WU Z Z,et al.Effects of rumen-protected folic acid and rumen-protected sodium selenite supplementation on lactation performance,nutrient digestion,ruminal fermentation and blood metabolites in dairy cows[J].J Sci Food Agric,2019, 99(130):5826-5833.
[32]	ZHANG Z,LA S K,ZHANG G W,et al.Diet supplementation of palm fat powder and coated folic acid on performance,energy balance,nutrient digestion,ruminal fermentation and blood metabolites of early lactation dairy cows[J].Anim Feed Sci Technol, 2020, 265:114520.
[33]	DUPLESSIS M,LAPIERRE H,PELLERIN D,et al.Effects of intramuscular injections of folic acid,vitamin B12,or both,on lactational performance and energy status of multiparous dairy cows[J].J Dairy Sci,2017,100(5):4051-4064.
[34]	VANACKER N,GIRARD C L,BLOUIN R,et al.Effects of feed restriction and supplementary folic acid and vitamin B12 on immune cell functions and blood cell populations in dairy cows[J].Animal,2020,14(2):339-345.
[35]	LI S Z,ZHI L H,LIU Y L,et al.Effect of in ovo feeding of folic acid on the folate metabolism,immune function and epigenetic modification of immune effector molecules of broiler[J].Br J Nutr,2016,115(3):411-421.
[36]	FENG D,ZHOU Y,XIA M,et al.Folic acid inhibits lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages by suppressing MAPKs and NF-κB activation[J].Inflamm Res,2011,60(9):817-822.
[37]	SAMBLAS M,MARTÍNEZ J A,MILAGRO F.Folic acid improves the inflammatory response in LPS-activated THP-1 macrophages[J].Mediators Inflamm,2018,2018:1312626.
[38]	LA S K,LI H,WANG C,et al.Effects of rumen-protected folic acid and dietary protein level on growth performance,ruminal fermentation,nutrient digestibility and hepatic gene expression of dairy calves[J].J Anim Physiol Anim Nutr,2019,103(4):1006-1014.
[39]	OUATTARA B,BISSONNETTE N,DUPLESSIS M,et al.Supplements of vitamins B9 and B12 affect hepatic and mammary gland gene expression profiles in lactating dairy cows[J].BMC Genomics,2016,17(1):640.
[40]	MURNEY R,STELWAGEN K,WHEELER T T,et al.The effects of milking frequency in early lactation on milk yield,mammary cell turnover,and secretory activity in grazing dairy cows[J].J Dairy Sci,2015,98(1):305-311.
[41]	CAMPBELL K S,PURDY A K.Structure/function of human killer cell immunoglobulin-like receptors:lessons from polymorphisms,evolution,crystal structures and mutations[J].Immunology,2011,132(3):315-325.
[42]	CORY S,ADAMS J M.The Bcl2 family:regulators of the cellular life-or-death switch[J].Nat Rev Cancer,2002,2(9):647-656.
[43]	WARNER N,BURBERRY A,FRANCHI L,et al.A Genome-wide siRNA screen reveals positive and negative regulators of the NOD2 and NF-kB signaling pathways[J].Sci Signal,2013,6(258):rs3.

The Effect of Folic Acid Supplementation on the Expression of Genes Related to Immunity and Milking Traits in Subclinical Mastitis Cows

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 43

Related Articles 13

Recommended Articles

Metrics

Comments

[1]	YANG Yang, ZHOU Ziwei, ZHANG Jingyi, YANG Shuo, WANG Boyu, GE Nan, LIN Ye, HOU Xiaoming. Analysis on SP1 Gene Structure and Its Function on Milk Fat Synthesis in Holstein Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2970-2981.
[2]	LIU Jiamin, YU Baojun, MU Tong, ZHANG Di, FENG Xiaofang, ZHANG Juan, WANG Ying, WEN Wan, GU Yaling. Screening and Identification of Key miRNAs for Milk Fat Metabolism in Dairy Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4244-4257.
[3]	LIANG Yan, WANG Haiyang, GUO Mengling, ZHANG Qiang, GAO Qisong, LI Mingxun, ZHANG Huimin, YANG Zhangping, MAO Yongjiang. Effects of the Number of Subclinical Mastitis Infection during First 60 Days after Calving on SCS of Lactation Months of Holstein Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 352-363.
[4]	SHI Liang-yu, ZHANG Rui-qiang, LI Xiang, Serafino Musa Abdelkarim Augustino, Muhammad Zahoor Khan, LIU Lin, XIAO Wei, TANG Shao-qing, ZHANG Yi, WANG Ya-chun, YU Ying. Heritability Estimation of SCS Difference and Its Variation in Chinese Holstein Cattle in Beijing [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 935-941.
[5]	YIN Bin, YUE Shu-jian, YU Ying, CAO Qiao-qiao, WANG Zhong-hua, SHI Ke-rong. Selection of Microsatellite Markers Used for Pedigree Tree Construction and/or Paternity Testing in Chinese Holstein Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 595-604.
[6]	SONG Min-yan，WEI Yi-yuan，Muhammad Zahoor Khan，WANG Xin，YU Ying. Molecular Marker Study of Inflammatory Reaction in Bovine Mammary Epithelium Cell Line Induced by Methicillin-Resistant Staphylococcus aureus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1995-2002.
[7]	GAN Zong-hui, YANG Zhang-ping, LI Yun-long, MAO Yong-jiang, LIU Xian-hui, CHEN Fei. Relationship of Bacterial Infection with Somatic Cell Count and Milk Composition in Dairy Cows with Mastitis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 972-979.
[8]	ZHANG Mei-rong, CHEN Dan, LIAO Xiang-xiang, MAO Yong-jiang, LIU Kun, CHEN Liang, WANG Xing-long, YANG Zhang-ping, YANG Li-guo. Genetic Polymorphism of IL8 Gene and Its Association with Milk Traits and SCS in Holstein Imported from Australia [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 690-696.
[9]	CHANG Ling-ling;YANG Zhang-ping;WU Hai-tao;CHEN Ying;SHI Xue-kui;MAO Yong-jiang;CEN Ning;LIANG Xiang-huan;YIN Zhao-hua . Comparative Study on Fatty Acid Composition between Normal Milk and Subclinical Mastitis Milk of Dairy Cow [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(1): 44-47.
[10]	YANG Yong-xin;CHENG Guang-long;ZHAO Hui-ling;JIANG Xi-chun;CHEN Sheng. Differential Proteomics Analysis of Plasma Protein from Escherichia Coli Infected and Clinical Healthy Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(9): 1191-1197.
[11]	MA Pei-pei;YU Ying;ZHANG Yuan;ZHANG Qin;WANG Ya-chun;SUN Dong-xiao;ZHANG Yi . The Distribution of SCC and Its Correlation with Milk Production Traits in Chinese Holsteins [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(12): 1529-1535.
[12]	MA Yan;JIA Jin;ZHANG Yi;SUN Dongxiao;ZHANG Yuan . Association between the Polymorphism of GHR and Milk Production Traits in Chinese Holstein Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(8): 1186-1190.
[13]	CHEN Tao;GAO Yanxia;CAO Yufeng;LI Jianguo;LI Weijun . Effects of SteamFlaked Corn on the Production Performance and Excretion of Nitrogen and Phosphorus in Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(12): 1769-1775.