外膜蛋白W与鸭源鸡杆菌抗渗透应激相关

doi:10.11843/j.issn.0366-6964.2020.04.019

Abstract

Abstract: This study was conducted to investigate the role of outer membrane protein W (OmpW)of Gallibacterium anatis(G. anatis)in osmotic stress resistance. The survival rate and growth of wild type G. anatis RZ(WT) and its ompW deletion mutant ΔompW under different NaCl concentrations were determined. The expression of outer membrane proteins of WT and ΔompW under different NaCl concentrations were analyzed by SDS-PAGE and Western blot. Furthermore, transcription of OmpW mRNA was determined by real time PCR. In this study, we found that deletion of ompW result in reduced G. anatis growth in hypersaline culture conditions. Moreover, compared with survival rate of the WT strain, the survival rate of the ΔompW was reduced significantly in BHI with 3.0% NaCl(95.17% vs 83.50%, P<0.05). Compared with normal NaCl concentration (0.5%), transcription (1.19×) and expression of OmpW gene were up-regulated in cultures containing high NaCl concentrations. This study demonstrated that OmpW is involved in osmotic stress resistance of G. anatis.

Key words: Gallibacterium anatis, outer membrane protein, osmotic stress resistance

CLC Number:

S852.612

PENG Zhifeng, YANG Xia, LIU Hongying, WANG Xinwei, CHANG Hongtao, CHEN Lu, WANG Chuanqing. The Outer Membrane Protein W of Gallibacterium anatis is Involved in Osmotic Stress Resistance[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 827-833.

References 36

[1]	CHRISTENSEN H, BISGAARD M, BOJESEN A M, et al. Genetic relationships among avian isolates classified as Pasteurella haemolytica, ‘Actinobacillus salpingitidis’ or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov[J]. Int J Syst Evol Microbiol, 2003, 53(1):275-287.
[2]	BISGAARD M, KORCZAK B M, BUSSE H J, et al. Classification of the taxon 2 and taxon 3 complex of Bisgaard within Gallibacterium and description of Gallibacterium melopsittaci sp. nov., Gallibacterium trehalosifermentans sp. nov. and Gallibacterium salpingitidis sp. nov[J]. Int J Syst Evol Microbiol, 2009, 59(4):735-744.
[3]	PAUDEL S, LIEBHART D, HESS M, et al. Pathogenesis of Gallibacterium anatis in a natural infection model fulfils Koch's postulates:1.Folliculitis and drop in egg production are the predominant effects in specific pathogen free layers[J]. Avian Pathol, 2014, 43(5):443-449.
[4]	王川庆, 陈陆, 杨霞, 等. 蛋鸡群卡氏杆菌感染情况的初步研究[J]. 河南农业科学, 2008(3):97-100, 103.WANG C Q, CHEN L, YANG X, et al. A premilinary study of Gallibacterium anantis infection in laying hens[J]. Journal of Henan Agricultural Sciences, 2008(3):97-100, 103. (in Chinese)
[5]	SINGH S V, SINGH B R, SINHA D K, et al. Gallibacterium anatis. an emerging pathogen of poultry birds and domiciled birds[J]. J Vet Sci Technol, 2016, 7(3):1000324, doi:10. 4172/2157-7579. 1000324.
[6]	郭伦涛. 鸡杆菌地方分离株耐药性及耐药基因的研究[D]. 郑州:河南农业大学, 2010.GUO L T. Studies on drug resistance and resistant genes of Gallibacterium anatis strains isolated from chickens in different localities[D]. Zhengzhou:Henan Agricultural University, 2010. (in Chinese)
[7]	彭志锋,高冬生,刘红英, 等.鸭源鸡杆菌整合子及其与耐药性的相关性分析[J].畜牧兽医学报, 2016, 47(8):1676-1681.PENG Z F, GAO D S, LIU H Y, et al. Gallibacterium anatis integron and its correlation with the drug resistance[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(8):1676-1681. (in Chinese)
[8]	BAGER R J, NESTA B, PORS S E, et al. The fimbrial protein FlfA from Gallibacterium anatis is a virulence factor and vaccine candidate[J]. Infect Immun, 2013, 81(6):1964-1973.
[9]	GARCÍA-GÓMEZ E, VACA S, PÉREZ-MÉNDEZ A, et al. Gallibacterium anatis-secreted metalloproteases degrade chicken IgG[J]. Avian Pathol, 2005, 34(5):426-429.
[10]	KRISTENSEN B M, FREES D, BOJESEN A M. Expression and secretion of the RTX-toxin GtxA among members of the genus Gallibacterium[J]. Vet Microbiol, 2011, 153(1-2):116-123.
[11]	李欢. 鸭源鸡杆菌流行株毒素蛋白GtxA表达及其部分生物学特性的研究[D]. 郑州:河南农业大学, 2014.LI H. The expression of protein GtxA and research of some biological characteristics of Gallibacterium anatis[D]. Zhengzhou:Henan Agricultural University, 2014. (in Chinese)
[12]	MONTES-GARCÍA J F, VACA S, VAZQUEZ-CRUZ C, et al. Identification of a Hemagglutinin from Gallibacterium anatis[J]. Curr Microbiol, 2016, 72(4):450-456.
[13]	RAMIREZ-APOLINAR S, GUERRA-INFANTE F M, HARO-CRUZ M D J D, et al. Characterization of a Gallibacterium genomospecies 2 hemagglutinin[J]. J Anim Vet Adv, 2012, 11(4):556-560.
[14]	BAGER R J, PERSSON G, NESTA B, et al. Outer membrane vesicles reflect environmental cues in Gallibacterium anatis[J]. Vet Microbiol, 2013, 167(3-4):565-572.
[15]	王艳, 王坤芃, 王继洋, 等. 鸭源鸡杆菌外膜蛋白A的原核表达及免疫保护性分析[J]. 中国兽医科学, 2017,47(10):1269-1274.WANG Y, WANG K P, WANG J Y, et al. Prokaryotic expression of Gallibacterium anatis OmpA and immune protection evaluation of the expression product[J]. Chinese Veterinary Science, 2017,47(10):1269-1274. (in Chinese)
[16]	彭志锋, 崔丹丹, 杨霞, 等. 鸭源鸡杆菌ompW基因缺失菌株的构建及其药物敏感性分析[J]. 畜牧兽医学报, 2017, 48(7):1306-1313.PENG Z F, CUI D D, YANG X, et al. Construction of ompW gene deletion strain of Gallibacterium anatis and its susceotibility to antibacterial agents[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(7):1306-1313. (in Chinese)
[17]	HORST R, STANCZAK P, WVTHRICH K. NMR polypeptide backbone conformation of the E. coli outer membrane protein W[J]. Structure, 2014, 22(8):1204-1209.
[18]	XU C X, REN H X, WANG S Y, et al. Proteomic analysis of salt-sensitive outer membrane proteins of Vibrio parahaemolyticus[J]. Res Microbiol, 2004, 155(10):835-842.
[19]	MAITI B, RAGHUNATH P, KARUNASAGAR I, et al. Cloning and expression of an outer membrane protein OmpW of Aeromonas hydrophila and study of its distribution in Aeromonas spp.[J]. J Appl Microbiol, 2009, 107(4):1157-1167.
[20]	WU L N, LIN X M, WANG F P, et al. OmpW and OmpV are required for NaCl regulation in Photobacterium damsela[J]. J Proteome Res, 2006, 5(9):2250-2257.
[21]	YE Y W, LING N, GAO J N, et al. Roles of outer membrane protein W (OmpW) on survival, morphology, and biofilm formation under NaCl stresses in Cronobacter sakazakii[J]. J Dairy Sci, 2018, 101(5):3844-3850.
[22]	ZHANG X Y, GAO J N, LING N, et al. Short communication:roles of outer membrane protein W on survival, cellular morphology, and biofilm formation of Cronobacter sakazakii in response to oxidative stress[J]. J Dairy Sci, 2019, 102(3):2017-2021.
[23]	NANDI B, NANDY R K, SARKAR A, et al. Structural features, properties and regulation of the outer-membrane protein W (OmpW) of Vibrio cholerae[J]. Microbiology, 2005, 151(9):2975-2986.
[24]	郑鹿平. 我国鸡群鸡杆菌的首次分离鉴定及生物学特性初步研究[D]. 郑州:河南农业大学, 2010.ZHENG L P. The first isolation and identification of Gallibacterium anatis from fowls in china and preliminary research on its biological Characterics[D]. Zhengzhou:Henan Agricultural University, 2010. (in Chinese)
[25]	HUANG J C, WANG X R, CAO Q, et al. ClpP participates in stress tolerance and negatively regulates biofilm formation in Haemophilus parasuis[J]. Vet Microbiol, 2016, 182:141-149.
[26]	KIM S W, CHOI C H, MOON D C, et al. Serum resistance of Acinetobacter baumannii through the binding of factor H to outer membrane proteins[J]. FEMS Microbiol Lett, 2009, 301(2):224-231.
[27]	LIN J, HUANG S X, ZHANG Q J. Outer membrane proteins:key players for bacterial adaptation in host niches[J]. Microbes Infect, 2002, 4(3):325-331.
[28]	BEKETSKAIA M S, BAY D C, TURNER R J. Outer membrane protein OmpW participates with small multidrug resistance protein member EmrE in quaternary cationic compound efflux[J]. J Bacteriol, 2014, 196(10):1908-1914.
[29]	HATFALUDI T, AL-HASANI K, BOYCE J D, et al. Outer membrane proteins of Pasteurella multocida[J]. Vet Microbiol, 2010, 144(1-2):1-17.
[30]	HONG H, PATEL D R, TAMM L K, et al. The outer membrane protein OmpW forms an eight-stranded β-barrel with a hydrophobic channel[J]. J Biol Chem, 2006, 281(11):7568-7577.
[31]	FU X P, ZHANG J Y, LI T Y, et al. The outer membrane protein OmpW enhanced V. cholerae growth in hypersaline conditions by transporting carnitine[J]. Front Microbiol, 2018, 8:2703, doi:10. 3389/fmicb. 2017. 02703.
[32]	CAI S J, INOUYE M. EnvZ-OmpR interaction and osmoregulation in Escherichia coli[J]. J Biol Chem, 2002, 277(27):24155-24161.
[33]	HERNÁNDEZ-ALLÉS S, ALBERTI S, ÁLVAREZ D, et al. Porin expression in clinical isolates of Klebsiella pneumoniae[J]. Microbiology, 1999, 145(3):673-679.
[34]	JOVANOVICH S B, MARTINELL M, RECORD M T Jr, et al. Rapid response to osmotic upshift by osmoregulated genes in Escherichia coli and Salmonella typhimurium[J]. J Bacteriol, 1988, 170(2):534-539.
[35]	KAPFHAMMER D, KARATAN E, PFLUGHOEFT K J, et al. Role for glycine betaine transport in Vibrio cholerae osmoadaptation and biofilm formation within microbial communities[J]. Appl Environ Microbiol, 2005, 71(7):3840-3847.
[36]	GIL F, IPINZA F, FUENTES J, et al. The ompW (porin) gene mediates methyl viologen (paraquat) efflux in Salmonella enterica serovar typhimurium[J]. Res Microbiol, 2007, 158(6):529-536.

The Outer Membrane Protein W of Gallibacterium anatis is Involved in Osmotic Stress Resistance

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