甜菜碱对瘦素缺陷小鼠肌肉组织中脂质沉积的影响初探

doi:10.11843/j.issn.0366-6964.2020.01.012

Abstract

Abstract: To investigate the effect of betaine on lipid deposition and its potential mechanism in muscle tissue of mice with leptin deficiency (ob/ob), in this study, twelve 6-week-old healthy ob/ob male mice of the same batch were randomly divided into two groups:experimental group and control group. Their weight difference was less than 1 g. Mice in the control group drank purified water, and mice in the experimental group drank purified water containing 1% betaine. One month later, the mice were killed and muscle samples were collected. Then, lipid deposition in muscles was detected by oil red O staining and triglyceride detection, and fatty acids composition and content in muscles were analyzed by Meteorological Chromatography-Mass Spectrometry System. Also, qRT-PCR was used to measure gene expression levels in muscle. The results showed that,compared with control group:1) The weight gain, lipids deposition and fasting blood glucose were significantly reduced after ob/ob mice were treated with betaine(P<0.05). 2) Betaine supplementation caused the extremely significantly increase in the expression of hormone-sensitive lipase (HSL), adipose triacylglyceride lipase (ATGL) and glucose transpoter 4 (GLUT4) (P<0.01). 3) Betaine supplementation could significantly increase saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) content in muscle tissues of ob/ob mice(P<0.05). 4) Betaine supplementation significantly down regulated the expression of fatty acids synthetase (FAS, P<0.05), and up regulated the expression of fatty acid oxidation genes such as peroxisome proliferator activated receptor alpha (PPARα), acyl-CoA oxidase2 (ACOX2), long chain acyl-CoA dehydrogenase (ACADL) and fatty acid transposase (CD36) in muscle of ob/ob mice(P<0.01), while no significant difference was observed in acetyl-CoA synthetase (ACS) expression levels between experimental group and control group(P>0.05). When ob/ob mice were supplemented with betaine for one month, the expression of fatty acid synthesis gene in muscle tissue decreased, the expression of fatty acid oxidation gene increased, and the fatty acid content of muscle tissue changed, which eventually led to the decrease of muscle tissue lipid.

Key words: betaine, ob/ob mice, muscle tissue, lipid deposition

CLC Number:

S816

HE Jin, ZHANG Peiwen, DU Jingjing, XIAO Junsen, YANG Dongli, WANG Dingguo, ZHANG Shunhua, ZHU Li. Preliminary Study on the Effect of Betaine on Lipid Deposition in Muscle Tissue of Mice with Leptin Deficiency[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 99-108.

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Preliminary Study on the Effect of Betaine on Lipid Deposition in Muscle Tissue of Mice with Leptin Deficiency

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