福美双诱导的肉鸡胫骨软骨发育不良软骨细胞凋亡

doi:10.11843/j.issn.0366-6964.2017.12.023

Abstract

Abstract:

In order to investigate the region of apoptosis and the expression of genes related to apoptosis in cartilage cells of Tibial Dyschondroplasia (TD) in broiler chickens, TD model was established, in which eighty AA broiler chickens were divided into two groups; control group and experiment group (given thiram 100 mg·kg^-1 in feed). The morphology of chondrocytes and chondrocytes apoptosis in tibial growth plate of broilers were determined by HE (hematoxylin and eosin) staining and TUNEL technology. Real-time PCR and immunohistochemistry respectively were used to detect the mRNA transcription level of apoptosis-related genes in chondrocytes and protein expression levels. The HE staining result revealed that obvious cartilage plug formed and gradually bigger in the tibial growth plate after day 1, 2, 4 and 6 in TD broilers induced by thiram; and the abnormity of chondrocyte also increased. The TUNEL technology result showed that apoptosis levels of the chondrocytes significantly increased in cartilage lesion parts, especially in the hypertrophic zone. The Real-time PCR results showed that the mRNA transcription level of Bax was up-regulated from day 1 to 6 significantly (P<0.01). The Caspase-3 gene transcription significantly increased on day 1, 2 and 4 in TD broiler tibial growth plate (P<0.01). The transcription of anti-apoptosis gene BAG-1 was significantly decreased on day 1 and 2 in TD broiler tibial growth plate (P<0.01). The results of immunohistochemistry showed that the expression of Bax protein was significantly higher in the treated group than in the control group on day 1 and 4. It is concluded that the apoptosis of chondrocytes in TD broilers growth plate was related to the enhancement of pro-apoptotic gene expression and decreased of anti-apoptotic gene expression.

CLC Number:

S852.34

ZHANG Ning, LI Xin, NING Guan-bao, ZHANG Ding, Ali Raza Jahejo, LI Hong-quan, MA Hai-li, HAO Wei-fang, GAO Wen-wei, ZHAO Yu-jun, GAO Shi-min, LI Gui-lan, LI Jian-hui, YAN Fang, GAO Rong-kun, TIAN Wen-xia. Apoptosis of Chondrocytes in Tibial Dyschondroplasia Induced by Thiram in Broilers[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2421-2428.

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Apoptosis of Chondrocytes in Tibial Dyschondroplasia Induced by Thiram in Broilers

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