热应激致Hsc70出入核与细胞凋亡的关系

doi:10.11843/j.issn.0366-6964.2017.12.022

Abstract

Abstract:

The experiment was conducted to study effects of heat stress on Hsc70 import-export nuclear and apoptosis in H9c2 cardiac myocytes. H9c2 cells were exposed to 42℃ as the heat stress model, inhibited expression of Hsc70 was performed by transfecting Hsc70 siRNA, the expression of Hsc70 in the cytoplasm and nucleus were detected by Western blot, LDH concentration of cell culture was detected by ELISA, and apoptosis was detected by Annexin V-FITC/PI double staining. The results showed that:in normal H9c2 cardiomyocytes, the level of Hsc70 in cytoplasm was high and that in nucleus was low, the level of Hsp72 in cytoplasm and nucleus was very low. There was no significant difference in the expression of Hsc70 after heat stress. The expression of Hsc70 in nucleus was significantly increased after heat stress 30 and 100 min (P<0.01), and decreased after heat stress 240 min. After heat stress, the expression of Hsp72 was significantly increased (P<0.01 or P<0.05). After Hsc70 inhibited, the level of cytosolic Hsc70 was significantly decreased, and Hsc70 import nucleus decreased significantly after heat stress. After Hsc70 inhibited, there was no significant effect on the expression of Hsp72 in cytoplasm and nucleus. Compared with the heat stress group, the expression of LDH was increased in the heat stress+Hsc70 siRNA group, and there was a significant difference between the two groups after heat stress 100 min (P<0.05). After Hsc70 inhibited, H9c2 cells were more prone to apoptosis after heat stress, and there was a significant difference between the two groups in heat stress 30 and 100 min (P<0.05). In conclusion, heat stress can cause Hsc70 import and export nuclear, and the low level of Hsc70 nucleus can cause of H9c2 cells damage aggravated and the rate of apoptosis increased after heat stress.

CLC Number:

S852.33

CHEN Hong-bo, DUAN Dian-ning, BAO En-dong. The Relationship between Hsc70 Import-export Nuclear and Apoptosis by Heat Stress[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2414-2420.

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The Relationship between Hsc70 Import-export Nuclear and Apoptosis by Heat Stress

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