叶酸对炔雌醚致雄性大鼠生殖损伤的保护作用研究

doi:10.11843/j.issn.0366-6964.2017.02.019

Abstract

Abstract:

The study was conducted to investigate the effects of folacin on quinestrol induced abberant reproduction in adult male rat. Twenty 8-week-old adult male SD rats were randomly divided into 4 groups after 1-week of adaptive feeding. Control group: olive oil + physiological saline; Quinestrol group: 1.0 mg•kg^-1 quinestrol; Quinestrol + folacin group: 1.0 mg•kg^-1 quinestrol + 1.1 mg•kg^-1 folacin (dissolved in saline); Folacin group: 1.1 mg•kg^-1 folacin. Quinestrol and folacin were given intragastric administration to male rats daily for 2 weeks. After the treatments, the sexual organs were weighed, semen quality were tested, antioxidant status of testes were investigated, testis tissue section were made, histopathology of testes were observed, the expressions of Caspase-3 and endothelial nitric oxide synthase (eNOS) were detected by immunohistochemical staining. The results showed that atrophy of sexual organs were induced, the weights of sexual organs were decreased, abnormal spermatogenesis were abberant, sperm quality were reduced, antioxidant enzyme activity were decreased, oxidative stress were happend in testes after the treatment of quinestrol alone. By contrast, the expressions of Caspase-3 and eNOS in the testes were increased. In the group of quinestrol and folacin treatment, oxidative stress were reduced by decreasing eNOS expression, the expression of Caspase-3 were inhibited, the sperm deformity rate and the damage of the tubule were reduced, the reproductive function of male rats were improved significantly. Compared with the control group, the testicular antioxidant enzyme activities, tissue structure and sperm quality were ameliorated to some extent in the folacin treatment group, there was no significant difference. The results clearly demonstrate for the first time that folacin effectively inhibited the damaging effects of quinestrol on reproductive function in adult male rats.

CLC Number:

S85
S814

LI Jian, CHEN Fu-ning, CHEN Yao-xing, WANG Zi-xu, DONG Yu-lan, CAO Jing,SI Li-fang,LIU Zhi-jun,ZHAO Zhan-qin. The Study of Protection of Folacin against Quinestrol Induced Reproductive Toxicity in Male Rat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 351-358.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.02.019

https://www.xmsyxb.com/EN/Y2017/V48/I2/351

References

［1］TANG T, QIAN K, SHI T, et al. Photodegradation of quinestrol in waters and the transformation products by UV irradiation［J］. Chemosphere, 2012. 89:1419-1425.
［2］ZHAO M, LIU M, LI D, et al. Anti-fertility effect of levonorgestrel and quinestrol in Brandt’s voles (Lasiopodomys brandtii) ［J］. Integr Zool, 2007, 2(4):260-268.
［3］LÜ X, GUO Y, SHI D. Effects of quinestrol on reproductive hormone expression, secretion, and receptor levels in female Mongolian gerbils (Meriones unguiculatus) ［J］.Theriogenology, 2012, 77(6):1223-1231.
［4］SHEN W, SHI D, WANG D, et al. Inhibitive effects of quinestrol on male testes in Mongolian gerbils (Meriones unguiculatus) ［J］. Res Vet Sci, 2012, 93(2): 907-913.
［5］LI J, CHEN F, LI C, et al. Quinestrol induces spermatogenic apoptosis in vivo via increasing pro-apoptotic proteins in adult male mice［J］. Tissue Cell, 46(5): 318-325.
［6］HEMACHAND T, SHAHA C. Functional role of sperm surface glutathione S-transferases and extra cellular glutathione in the haploid spermatozoa under oxidative stress［J］.FEBS Lett, 2003, 538(1):14-18.
［7］AGARWAL A, SALEH R A, BEDAIWY M A. Role of reactive oxygen species in the pathophysiology of human reproduction［J］. Fertil Steril, 2003, 79(4):829-843.
［8］SUBUDHI U, DASA K, PAITAL B, et al. Alleviation of enhanced oxidative stress and oxygen consumption of L-thyroxine induced hyperthyroid rat liver mitochondria by vitamin E and curcumin［J］. Chem Biol Interact, 2008, 173(2):105-114.
［9］BURTON G J, JAUNIAUX E. Oxidative stress［J］. Best Pract Res Clin Obstet Gynaecol, 2011, 25(3):287-299.
［10］VAANHOLT L M, MILNE A, ZHENG Y, et al. Oxidative costs of reproduction: Oxidative stress in mice fed standard and low antioxidant diets［J］. Physiol Behav, 2016, 154:1-7.
［11］PLANTAMP C, SALORT K, GIBERT P, et al. All or nothing: Survival, reproduction and oxidative balance in Spotted Wing Drosophila (Drosophila suzukii) in response to cold［J］. J Insect Physiol, 2016, 8:28-36.
［12］刘艳利，申静，支丽慧，等．叶酸调控鸡脾和胸腺IGF-2表达的表观遗传机制探究［J］．畜牧兽医学报，2016，47(2):296-304.
LIU Y L, SHEN J, ZHI L H, et al. The study on epigenetic mechanism of IGF2 expression in spleen and thymus regulated by folic acid in broilers［J］. Acta Veterinaria et Zootechnica Sinica, 2016, 47(2):296-304.(in Chinese)
［13］KAMEN B. A review on folate and antifolate pharmacology［J］. Semin Oncol, 1997, 24 (5):18-39.
［14］SHALABY M A, EL ZORBA H Y, ZIADA R M. Reproductive toxicity of methomyl insecticide in male rats and protective effect of folic acid［J］. Food Chem Toxicol, 2010, 48(11):3221-3226.
［15］CAILLE N, RODINA M, KOCOUR M, et al. Quantity, motility and fertility of tench Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments［J］. Aqua Int, 2006, 14(1):75-87.
［16］SÖNMEZ M, TÜRK G, YÜCE A. The effect of ascorbic acid supplementation on sperm quality, lipid peroxidation and testosterone levels of male Wistar rats［J］.Theriogenology, 2005, 63(7): 2063-2072.
［17］WATSON P F. Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa［J］. Vet Rec, 1975, 97(1):12-15.
［18］MA A, YANG X, WANG Z, et al. Adult exposure to diethylstilbestrol induces spermatogenic cell apoptosis in vivo through increased oxidative stress in male hamster［J］. Reprod Toxicol, 2008,25:367-373.
［19］JURAND A, GOEL S C. The use of methyl green-pyronin staining after glutaraldehyde fixation and paraffin or araldite embedding ［J］. Tissue Cell, 1976, 8(2):389-394.
［20］李健，陈耀星，陈福宁，等．MAPK 信号通路在炔雌醚诱导大鼠生精细胞凋亡中的作用研究［J］．畜牧兽医学报，2016，47(2): 381-387.
LI J, CHEN Y X, CHEN F N, et al. The role of MAPK signaling pathway in quinestrol-induced apoptosis of spermatogenic cells in rat［J］. Acta Veterinaria et Zootechnica Sinica, 2016, 47(2): 381-387.(in Chinese)
［21］CAI L, CHEN S, EVANS T, et al. Apoptotic germ-cell death and testicular damage in experimental diabetes: prevention by endothelin antagonism［J］. Urol Res, 2000, 28(5):342-347.
［22］CAMERON D F, MURRAY F T, DRYLIE D D. Interstitial compartment pathology and spermatogenic disruption in testes from impotent diabetic men［J］. Anat Rec, 1985, 213(1):53-62.
［23］BAN Y, KOMATSU T, KEMI M, et al. Testicular spermatid and epididymal sperm head counts as an indicator for reproductive toxicity in rats［J］. Exp Anim, 1995, 44(4):315-322.
［24］FENECH M. Micronutrients and genomic stability: a new paradigm for recommended dietary allowances (RDAs) ［J］. Food Chem Toxicol, 2002, 40(8):1113-1117.
［25］PADMANABHAN S, TRIPATHI D N, VIKRAM A, et al. Methotrexate-induced cytotoxicity and genotoxicity in germ cells of mice: Intervention of folic and folinic acid［J］. Mutat Res, 2009, 673(1):43-52.
［26］IBRAHIM W, TOUSSON E, MM ALI E, et al. Folic acid alleviates oxidative stress and hyperhomocysteinemia involved in testicular dysfunction of hypothyroid rats［J］. Gen Comp Endocr, 2011, 174(2) :143-149.
［27］SWAYNE B G, KAWATA A, BEHAN N A, et al. Investigating the effects of dietary folic acid on sperm count, DNA damage and mutation in Balb/c mice［J］. Mutat Res, 2012, 737(1): 1-7.
［28］MYAR C A, INGERSOLL R, WALLOCK L M. Folate levels and the effects of folate deficiency on the reproductive organs of male rats［J］. FASEB J, 1999, 13:229-232.
［29］FORGES T, MONNIER-BARBARINO P, ALBERTO J M, et al. Impact of folate and homocysteine metabolism on human reproductive health［J］. Hum Reprod Update, 2007, 13(3):225-238.
［30］LI J, WANG H, ZHANG J, et al. Abnormal secretion of reproductive hormones and antioxidant status involved in quinestrol induced reproductive toxicity in adult male rat［J］. Tissue Cell, 2014, 46(1): 27-32.
［31］STANGER O. Physiology of folic acid in health and disease［J］. Curr Drug Metab, 2002, 3(2):211-223.
［32］DEGTEREV A, BOYCE M, YUAN J. A decade of caspases［J］. Oncogene, 2003, 22(53):8543-8567.
［33］ZABIHI S, ERIKSSON U J, Wentzel P. Folic acid supplementation affects ROS scavenging enzymes, enhances Vegf-A, and diminishes apoptotic state in yolk sacs of embryos of diabetic rats［J］. Reprod Toxicol, 2007, 23(4):486-498
［34］李运书，孟祥硕，崔策，等．叶酸对胚胎大鼠心肌细胞凋亡及Bax、Bcl-2表达的影响［J］．承德医学院学报，2015, 3(14):259-261.
LI Y S, MENG X S, CUI C, et al. Effect of folic acid on cardiomyocyte apoptosis and the expressions of Bcl-2 and Bax in Embryonic rat［J］. Journal of Chengde Medical College, 2015, 3(14):259-261.(in Chinese)

[1]	CHONG Qian, ZHAO Xuehui, CAO Qing, XUE Huiwen, GOU Huitian. Research Progress of Applied Research on Listeria monocytogenes Phage [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1819-1826.
[2]	HAN Fuzhen, CAI Limeng, LI Zhuoran, WANG Xueying, XIE Weichun, KUANG Hongdi, LI Jiaxuan, CUI Wen, JIANG Yanping, LI Yijing, SHAN Zhifu, TANG Lijie. Research Progress on the Mechanism of Intestinal Flora-Mediated Regulation of Intestinal Mucosal Immunity by Secondary Bile Acids and Their Receptors [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1904-1913.
[3]	YU Zuhua, GAO Mengru, QI Zhiying, ZHANG Jingyu, HE Lei, CHEN Jian, DING Ke. Research Progress on the Function of RNA Binding Protein ELAVL1 and Its Regulation of Viral Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1914-1925.
[4]	HUANG Jin, LI Siyuan, MAO Li, CAI Xuhang, XIE Lingling, WANG Fu, ZHOU Hua, LI Jizong, LI Bin. Eukaryotic Expression of Bovine Coronavirus S1 Protein and Establishment and Application of Indirect ELISA [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2050-2060.
[5]	HAN Yang, GUAN Shuaiyin, LI Zhen, ZHOU Saisai, YUAN Honggen, SONG Yunfeng. Prokaryotic Expression and Protein Activity of Porcine Circovirus Type 3 Rep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2061-2071.
[6]	SONG Xiaoqing, DENG Ruide, LI Xin, LI Jiao, LI Runcheng, DU Lifei, DONG Wei, GE Meng. Establishment of ELISA for Detection of PCV4-Cap Antibody and Sero-epidemiological Survey [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2072-2079.
[7]	ZHOU Yang, WU Weizi, CAO Weisheng, WANG Fuguang, XU Xiuqiong, ZHONG Wenxia, WU Liyang, YE Jian, LU Shousheng. A Whole Genome Sequencing Method for African Swine Fever Virus based on Nanopore Sequencing Technology was Established [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2080-2089.
[8]	MA Rumeng, ZHAO Yuliang, MA Mingshuang, GUO Guihai, LIU Xinzi, LI Jiaxuan, CUI Wen, JIANG Yanping, SHAN Zhifu, ZHOU Han, WANG Li, QIAO Xinyuan, TANG Lijie, WANG Xiaona, LI Yijing. Comparative Study on the Immune Response Induced by the Different Porcine Receptor Bacteria with Expressing the Protective Antigen S1 of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2090-2099.
[9]	XU Hong, SHANG Hongqi, ZHANG Xue, QIAN Jiali, WANG Chuanhong, SONG Xu, BAO Meiying, LIU Shiyu, ZHANG Gege, GUO Rongli, ZHAO Yongxiang, FAN Baochao, LI Bin. Inhibition Effect of C8orf4 Gene Encoding Protein on in vitro Replication of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2100-2108.
[10]	XIONG Ting, HE Xianming, ZHAO Xiya, ZHUANG Tingting, HUANG Meizhen, LIANG Shijin, YU Chuanzhao, LIANG Xuejing, CHEN Ruiai. Whole Genome Analysis of Three Predominant Epidemic Strains of Chicken Infectious Bronchitis Virus and Their Pathogenicity [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2109-2122.
[11]	LÜ Yadi, YANG Jie, XIE Wenting, XU Ting, CHEN Ruiai. Construction and Evaluation of the Immune Effect of Recombinant Genotype Ⅶ NDV Strain Co-expressing Membrane-bound and Water-soluble HA Protein of Avian Influenza Virus H9N2 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2123-2134.
[12]	XU Zhenyu, DENG Xiaoyu, WANG Yueli, SUN Can, WU Aodi, CAO Jian, YI Jihai, WANG Yong, WANG Zhen, CHEN Chuangfu. Biological Characteristics of Brucella abortus A19ΔBtpA Deletion Strain and Its Immunogenicity Study [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2135-2145.
[13]	ZHAO Canqi, FENG Yu, LÜ Lang, LI Yanjun, WEI Yulei, DING Jiabo, CHEN Xiang, JIANG Hui. Study on Purification of Bovine Brucellosis by Competitive ELISA and Indirect ELISA [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2146-2153.
[14]	LUO Ting, HAN Zhu, XU Yefen, CAI Lin, SUOLANG Sizhu, XU Jinhua, NIU Jiaqiang. Whole Genome Sequencing and Sequence Analysis on T10 of Mycoplasma bovis Strain from Yaks in Xizang [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2154-2167.
[15]	GAO Jie, LI Xiaocheng, MU Yang, ZHANG Hui, WEI Rong, LI Jie. Biological Characteristics and Immune Effect Evaluation of Outer Membrane Vesicles of Capsular Type B Pasteurella multocida [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2168-2175.

The Study of Protection of Folacin against Quinestrol Induced Reproductive Toxicity in Male Rat

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments